中国畜牧杂志2017，Vol.53Issue(10)：34-38,5.DOI:10.19556/j.0258-7033.2017-10-034
3个品种猪MEF2D基因启动子多态及其生物信息学分析
The Polymorphisms and Bioinformatics of Promoter Region ofMEF2D Gene in Congjiang Xiang Pig
摘要
Abstract
The Congjiang Xiangpigs,the commercial crossbred pigs as well as the Guizhou Zongdi Huapigs as research animals.were used to screen the gene MEF2D of 5'UTR and the SNPs sites in part first exon region by using DNA pool and PCR direct sequencing.Variety bioinformatics softwares were used to analyse the SNPs sites how to affect the core region of the promoter,transcription factors binding sites and CpG island.The results showed there were four SNPs sites in the 5'UTR regions of MEF2D,including A-511G,T-453A,T-242G and T-180A.It was identified that various new transcription factors binding sites emerged and some significant transcription factors binding sites disappeared nearby the region at T-453A and T-242G site.So we speculated that T-453A and T-242G site locating MEF2D'5'UTR have a deep effect on regulating the functional element of promoter.关键词
从江香猪/MEF2D基因/SNP/启动子/生物信息学Key words
Congjiang Xiang Pig/MEF2D gene/SNP/Promoter/Bioinformatics分类
农业科技引用本文复制引用
白优,张勇,陈祥,张雄,李俊,何琦,杨红,陆静..3个品种猪MEF2D基因启动子多态及其生物信息学分析[J].中国畜牧杂志,2017,53(10):34-38,5.基金项目
贵州大学引进人才科研项目[贵大人基合字2013(21)号] （21）
贵州省工程中心建设项目(黔科合农C字[2011]4022号) （黔科合农C字[2011]4022号）
