中国畜牧杂志2017，Vol.53Issue(10)：52-54,3.DOI:10.19556/j.0258-7033.2017-10-052
微流路精子分离器体外受精方法对奶牛体外受精效果的影响
Effect of MFSS-IVF Method on in vitro Fertilization of Dairy Cow
摘要
Abstract
In order to solve the problems of low monospermy and blastocyst rates in IVF with bovine sexed semen,a microfluidic sperm sorter (MFSS) device was used.Ovaries were collected from slaughtered dairy cow.After oocytes maturation in vitro,MFSS was used to fertilize in vitro with sorted semen of bull.The objective of this study was to use a microfluidic sperm sorter to isolate sexed semen for bovine IVF.The rate of monospermy (64.38％) and blastocyst (40.12％) was higher (P＜0.05) in the MFSS-IVF system than standard IVF (29.02％ and 24.55％,respectively).No significant difference (P＞0.05) were found between sperm penetration and cleavage.MFSS-IVF system can increase monospermy and blastocyst with bovine sexed semen,without influence sperm penetration and cleavage.关键词
微流路精子分离器/奶牛/性控精液/体外受精Key words
Microfluidic sperm sorter/Dairy cow/Sexed semen/IVF分类
农业科技引用本文复制引用
李井春,韩淑敏,张帆,李琦,孙思怡,李雁冰,魏国生..微流路精子分离器体外受精方法对奶牛体外受精效果的影响[J].中国畜牧杂志,2017,53(10):52-54,3.基金项目
国家自然科学基金项目(31402136) （31402136）
黑龙江省自然科学基金项目(C2017053) （C2017053）
黑龙江省农垦总局科研计划项目(HNK135-04-06、HNK135-04-02-03) （HNK135-04-06、HNK135-04-02-03）
黑龙江八一农垦大学博士启动基金(B2012-06) （B2012-06）
大庆市指导性科技计划项目(zd-2016-087) （zd-2016-087）
