中国烟草科学2017，Vol.38Issue(5)：13-17,31,6.DOI:10.13496/j.issn.1007-5119.2017.05.003
抗青枯内生细菌的筛选及其对烟草青枯病的防治效果
Selection of Endophytic Antagonistic Bacteria for Control of Tobacco Bacterial Wilt
摘要
Abstract
Tobacco bacterial wilt is a soil-borne bacterial disease caused by Ralstonia solanacearum, which is mainly harmful to the roots and stems of tobacco and seriously affects tobacco yield. Fifty-five endophytic bacterial strains were isolated from healthy tobacco planted in diseased soils. Among them, strains R-3, R-7 and R-9 had obvious antagonistic effects on R. solanacearum in plate antagonistic experiments. Based on gyrB gene sequence, R-3, R-7 and R-9 all belong to Bacillus velezensis. After heated at 115 ℃, the liquid fermentation of the 3 strains still had antibacterial activities, indicating that these strains produced heat resistant substances. Field experiments showed that R-3, R-7 and R-9 were all able to chronically colonize in the root and stem of tobacco, and their control efficiency against bacterial wilt were 55.72%, 48.34% and 50.71%, respectively.关键词
烟草青枯病/植物内生菌/贝莱斯芽胞杆菌/定殖能力/防治效果Key words
tobacco bacterial wilt/plant endophytic bacteria/bacillus velezensis/colonization/control efficiency分类
农业科技引用本文复制引用
姜乾坤,彭阁,王瑞,谭军,邸慧慧,向必坤,赵秀云,彭五星,董善余..抗青枯内生细菌的筛选及其对烟草青枯病的防治效果[J].中国烟草科学,2017,38(5):13-17,31,6.基金项目
中国烟草总公司科技重点项目"基于宏基因组学的植烟土壤健康评价研究与应用"(110201402016) （110201402016）
