中国烟草科学2017，Vol.38Issue(5)：87-92,6.DOI:10.13496/j.issn.1007-5119.2017.05.015
贵州烤烟糖苷类化学成分抗氧化活性及分离鉴定
Antioxidant Activity and Active Component Identification of Glycosides from Leaves of Guizhou Flue-cured Tobacco
摘要
Abstract
To explore the chemical constituents of tobacco glycosides and their antioxidant activities, crude glycoside extracts from Guizhou flue-cured tobacco leaves were obtained by methanol-reflux and macroporous resin adsorption. DPPH radical-scavenging activities of different extracts of crude glycoside were investigated. Further separation and purification of ethyl acetate fractions were processed through reversed phase chromatography, gel chromatography, preparative liquid chromatography and thin layer chromatography. The results showed that, each crude glycoside extract showed certain DPPH radical-scavenging activities, in which the ethyl acetate fraction exhibited the best activity. Multiple monomeric compounds were isolated from the ethyl acetate fraction, and four glycoside chemical compounds were identified by NMR. The four monomers are 7,8-dihyd-3-oxo-α-ionol-β-D-glucopyranoside(1), 3-oxo-α-ionol-β-D-glucopyranosid (2), 6-O-β-D-glucopyranosid(3) and O-β-D- glucopyranosid(4).关键词
烟草/糖苷/分离纯化/DPPH/结构鉴定Key words
flue-cured tobacco/glycoside/separation and purification/DPPH/structure identification分类
轻工纺织引用本文复制引用
丁根胜,惠建权,耿召良,葛永辉,杨超,李永青,王承福,黄新民,黄正虹,杨盛刚,万里兴..贵州烤烟糖苷类化学成分抗氧化活性及分离鉴定[J].中国烟草科学,2017,38(5):87-92,6.基金项目
中国烟草总公司贵州省公司重点科技计划项目"贵州烟叶中性致香物质研究"(2013-07) （2013-07）
贵州中烟工业有限责任公司科技项目"贵州烟叶本源香提取物在卷烟配方中的应用研究"(GZZY/KJ/JS/2015CY014-1) （GZZY/KJ/JS/2015CY014-1）
