中国真菌学杂志2017，Vol.12Issue(5)：262-267,6.
人类免疫缺陷病毒阴性隐球菌性脑膜炎患者外周血淋巴细胞亚群分析
Immunophenotype of peripheral lymphocytes in HIV-negative patients with cryptococcal meningitis
摘要
Abstract
Objective To find if immunophenotypic features of peripheral lymphocytes was associated with risk of cryptococcal meningitis (CM) in HIV-negative individuals.Methods A total of 31 CM patients treated at a tertiary hospital in Beijing,China were included.They were divided into immunocompromised comorbidity group (n =16) and apparently immunocompetent group (n =15).Twenty-one healthy donors were included as control group.Lymphocyte subsets including B,NK,CD4 +T,and CD8+ T cells,CD28 expression on T cells,and activation markers (HLA-DR and CD38) on CD8+ T cells were determined by flow cytometry and compared among the three groups.Results Compared with healthy donors,immunocompromised comorbidity group had dramatically decreased median B cell (223 vs.56/μL),NK cell (280 vs.86/μL),CD4+ T cell (695 vs.218/μL),and CD8+T cell (521 vs.164/μL) counts (P values for all four subsets＜0.001).Proportions of activated CD8+ T cells were significantly increased in immunocompromised patients than that in healthy control.Except for CD8+ T cell counts (527/μL),the median B cell (128/μL,P=0.02),NK cell (128/μL,P=0.002) and CD4+ T cell (567/μL,P =0.03)were moderately reduced in the apparently immunocompetent group than those in the healthy donors.The levels of HLA-DR and CD38 on CD8+ T cells in the apparently immunocompetent group were similar with the healthy donors.CD28 expression on CD4+ T and CD8+ T cells did not differ among the three groups.Conclusions The immunophenotype of CM patients with immunocompromised comorbidity was characterized by generalized decreased B,NK,CD4+ T,and CD8+ T cells.Mild-to-moderate reduce in B cell,NK cell and CD4+ T cell counts were observed in apparently immunocompetent patients.关键词
脑膜炎,隐球菌性/淋巴细胞亚群/CD4+T细胞Key words
cryptococcal meningitis/lymphocyte subsets/CD4+ T cell分类
医药卫生引用本文复制引用
谢静,葛瑛,邱志峰,张凯宇,刘正印,李太生..人类免疫缺陷病毒阴性隐球菌性脑膜炎患者外周血淋巴细胞亚群分析[J].中国真菌学杂志,2017,12(5):262-267,6.基金项目
国家科技支撑计划课题(2014BAI07B06),北京协和医院中青年科研基金(pumch-2016-2.3) （2014BAI07B06）
