中国真菌学杂志2017，Vol.12Issue(5)：268-273,267,7.
新生隐球菌性脑膜炎患者外周血Th1/Th2及相关T淋巴细胞水平变化及意义
The proportions of Th1/Th2 and Relevant T lymphocytes in patients with cryptococcal meningitis and its clinical significance
黄煌 1冼莹 2潘越峻 1张扣兴2
作者信息
- 1. 广州市第八人民医院重症医学科,广州510060
- 2. 中山大学附属第三医院重症医学科,广州510630
- 折叠
摘要
Abstract
Objective This study is to further understanding the mechanism of T helper cells in course of C.neofermans infection from patient's level through measuring the proportions of Th1 /Th2 cell factors and NKT cells,γδT cells in peripheral blood between normal adult and patients.Analyze whether the change presents relevance with the disease prognosis.Method The peripheral blood samples were collected from 16 patients with cryptococcal meningitis,and 10 healthy people.The proportions of Th1/Th2 cell factors and NKT cells,γδ T cells were analyzed by flow cytometry (FCM).Results 1.The proportion of Th1 cells in CD4 +T lymphocyte of patients with cryptococcal meningitis before treatment was (13.093 ± 4.618)％,less than that of healthy people (19.582±4.949)％,P＜0.01.And the proportion of Th2 cells was (1.769±0.865)％,higher than that of healthy people (0.584±0.202) ％,P＜0.01.2.Patients who received treatment showed the increased levels of Th1 cells in CD4 +T lymphocyte (16.069 ± 1.516)％ which was higher than those in patients before treatment (13.589 ±2.101) ％,P＞0.05.The proportion of Th2 cells in the patients had received treatment was (1.499±0.274) ％,less than those of patients before treatment (1.673±0.290)％,P＞0.05.3.The proportion of NKT cells in T lymphocyte of patients with cryptococcal meningitis before treatment was (11.848±2.936)％,less than those of healthy people (17.918±8.088)％,P＜0.05.No significant differences of proportions of γ'δT in T lymphocyte among patients before treatment and healthy people[(9.166±3.989)％ vs (9.873±3.509)％],P＞0.05.4.Patients who received treatment showed the increased levels of NKT cells in T lymphocyte (14.806 ±1.513) ％ which was higher than those in patients before treatment (11.830±1.350)％,P＞0.05.The proportion of γδT cells in the patients who had received treatment was (6.984±0.843)％,less than those of patients before treatment (8.094±2.037)％,P＞0.05.Conclutions 1.The balance of Th1/Th2 falls towards Th2 and the decrease of the proportion of NKT cells among T lymphocytes in patients with cryptococcal meningitis.It suggests that the cell mediated immunity of patients with cryptococcal meningitis is weakened.2.Once the patients get better after treatment,Th1/Th2 imbalance would be corrected in that the balance turned to Th1 direction.It suggests that the recovery of cell-mediated immunity may improve the prognosis of these disease.关键词
新生隐球菌/感染/Th1/Th2/NKT/γδTKey words
Cryptococus neofermans/infections/Th1/Th2/NKT/γδT分类
医药卫生引用本文复制引用
黄煌,冼莹,潘越峻,张扣兴..新生隐球菌性脑膜炎患者外周血Th1/Th2及相关T淋巴细胞水平变化及意义[J].中国真菌学杂志,2017,12(5):268-273,267,7.
