中国肿瘤生物治疗杂志2017，Vol.24Issue(10)：1058-1062,5.DOI:10.3872/j.issn.1007-385x.2017.10.003
人肝癌微血管内皮细胞黏附分子表达特点及其对外周血单个核细胞黏附和跨内皮迁移的影响
Expression characteristics of adhesion molecules on HLCMVEC and its influence on adhesion and trans-endothelial migration of PBMC
摘要
Abstract
Objective:To investigate the expression characteristics of adhesion molecules on human liver cancer microvascular endothelial cells (HLCMVECs) and its influence on adhesion and trans-endothelial migration of immune cells.Methods:HLCMVECs were isolated and cultured,and human liver sinusoid endothelial cells (LSECs) were used as control.The expression of adhesion molecules was evaluated by cell-based ELISA.Peripheral blood mononuclear cells (PBMCs) were labeled with BCECF (a fluorescent dye) and then co-cultured with HLCMVEC or LSEC;the adhesion to endothelial cells and trans-endothelial migration of PBMCs were observed by inverted fluorescence microscope and continuous spectrum luminoscope.In addition,the functional antibody against each adhesion molecule was respectively added to endothelial cells before co-culture,which could determine the contribution of each adhesion molecule to the adhesion and trans-endothelial migration of PBMC.Results:Compared to LSECs,HLCMVECs expressed low level of CD31,CD34 and intercellular adhesion molecule-3 (ICAM-3) (P＜0.05) and an cven lower level of intercellular adhesion molecule-1 (ICAM-1) and Vascular cell adhesion molecule-1 (VCAM-1) (P＜0.01),but expressed significantly higher level of αv[β3 and αvβ5 (P＜0.01).Compared with LSECs,the adhesion of PBMCs to HLVMVECs were significantly decreased;and under the inducement of chemo-tactic agent,trans-endothelial migration of PBMCs through HLVMVECs were also significantly decreased (adhesion:[205.5 ± 46.0] vs [330.5 ± 48.4];migration:[49.0 ± 10.6] vs [110.0 ± 19.2];all P＜0.01).However,the adhesion and migration could be obviously blocked by antibodies against ICAM-3 (P＜0.05),ICAM-1 and VCAM-1(P＜0.01);Antibody against CD31 did not influence PBMC adhesion greatly but significantly reduced trans-endothelial migration (P＜0.05).Conclusion:The distinct expression of adhesion molecules on HLCMVECs reduced the adhesion and trans-endothelial migration of PBMCs.关键词
人肝癌微血管内皮细胞/黏附分子/外周血单个核细胞/黏附/迁移Key words
human liver cancer microvascular endothelial cell (HLCMVEC)/adhesion molecule/PBMC/adhesion/migration分类
医药卫生引用本文复制引用
朴秉国,翟敏,许世清,徐雅平,娄晋宁,刘虹麟,张文健..人肝癌微血管内皮细胞黏附分子表达特点及其对外周血单个核细胞黏附和跨内皮迁移的影响[J].中国肿瘤生物治疗杂志,2017,24(10):1058-1062,5.基金项目
国家自然科学基金资助项目(No.81402451,No.81370873),国家重点基础研究发展计划(973计划)资助项目(No.2009CB521804). Project supported by the National Natural Science Foundation of China (No.81402451,No.81370873),and the Major National Basic Research Development Program (973 Program) of China (No.2009CB521804) （No.81402451,No.81370873）
