中国肿瘤生物治疗杂志2017，Vol.24Issue(10)：1076-1080,5.DOI:10.3872/j.issn.1007-385x.2017.10.006
香加皮杠柳苷联用TRAIL对胃癌SGC-7901和MGC-803细胞的作用及其机制
Effect of combined treatment of cortex periplocae and TRAIL on gastric cancer SGC-7901 and MGC-803 cells and its mechanism
摘要
Abstract
Objective:To investigate the effect of combined treatment of cortex periplocin (CPP) and tumor necro sis factor related apoptosis inducing ligand (TRAIL) on gastric cancer cells and to explore the mechanism.Methods:After routine culture,the gastric cancer cell lines (SGC-7901 and MGC-803) were treated with CPP (at concentrations of 50,100,200 ng/ml) or TRAIL (1 μg/ml) or in combination of these two.The cell proliferation was detected by MTS,the apoptosis was detected by Flow cytometry,and the expression levels of pro-BID,Mcl-1,cleaved caspase-3,DR4 and DR5 were detected by Western blotting.Results:Compared with the control group and each CPP single treatment group,MTS assay demonstrated that CPP (50,100,200 ng/ml) in combination with TRAIL (1 μtg/ml) significantly inhibited the proliferation of gastric cancer SGC-7901 and MGC-803 cell lines (all P＜0.05 or P＜0.01).Flow cytometry demonstrated that the apoptosis rate of gastric cancer cells in combined treatment group was significantly higher than that of control group or each CPP single treatment group (P＜0.05 or P＜0.01).Western blotting demonstrated that combined treatment for 24 h significantly decreased the expression of pro-BID and Mcl-1 (P＜0.05),but increased the expression levels of cleaved caspase-3,DR4 and DR5(P＜0.05).Conclusion:CPP in combination with TRAIL could significantly induce the apoptosis of gastric cancer SGC-7901 and MGC-803 cell lines and increase the susceptibility of cancer cells to TRAIL.关键词
香加皮杠柳苷/肿瘤坏死因子相关凋亡诱导配体/胃癌细胞/增殖/凋亡Key words
cortex periplocae(CPP)/tumor necrosis factor related apoptosis inducing ligand (TRAIL)/gastric cancer cells/proliferation/apoptosis分类
医药卫生引用本文复制引用
孙佳玮,魏思思,董佩,李磊,戴素丽,赵连梅,单保恩..香加皮杠柳苷联用TRAIL对胃癌SGC-7901和MGC-803细胞的作用及其机制[J].中国肿瘤生物治疗杂志,2017,24(10):1076-1080,5.基金项目
国家科学自然基金项目资助(No.81673642). Project supported by the National Natural Science Foundation of China (No.81673642) （No.81673642）
