中国肿瘤生物治疗杂志2017，Vol.24Issue(10)：1081-1087,7.DOI:10.3872/j.issn.1007-385x.2017.10.007
辛二酰苯胺异羟肟酸对结肠癌HCT116和SW480细胞增殖、周期和凋亡的影响
Effects of suberoylanilide hydroxamic acid on proliferation, cell cycle and apoptosis of colon cancer HCT116 and SW480 cells
摘要
Abstract
Objective:To investigate the effects and molecular mechanisms of Suberoylanilide Hydroxamic Acid (SAHA) on the proliferation,cell cycle and apoptosis of colon cancer HCT116 and SW480 cells.Methods:Colon cancer cells (HCT116 and SW480) were treated with a series of concentrations of SAHA.MTT assay was employed to evaluate the effect of SAHA on proliferation of HCT116 and SW480 cells.The cell cycle distributionand apoptosis were determined by flow cytometry.Rhodamine 123 and DCFH-DA were applied to detect the mitochondrial membrane potential(A ψ m) and reactive oxygen species (ROS) production.Reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to assess the mRNA and protein expressions of AcH3,p21,Cyclin D1,Bax and Bcl-2.Results:After 48 h of SAHA treatment on HCT116 and SW480 cells,the cell proliferation was inhibited,the G1 phase ratio of cells and the apoptosis rate was increased (all P＜0.05);moreover,SAHA also reduced Aψm and increased cellular ROS production (all P＜0.05).Compared with control group,the mRNA expressions of p21 and Bax were higher in SAHA group,while the mRNA expressions of CyclinD1 and Bcl2 were lower (all P＜0.05);Furthermore,the protein expressions of Ac-H3,p21 and Bax were improved,while theprotein expressions of CyclinD1 and Bcl-2 were decreased in SAHA group (all P＜0.05).Conclusion:SAHA may suppress cell growth,induce apoptosis and cause cell cycle arrest of colon cancer cells by promoting histone acetylation and modulating their related genes of p21,CyclinD1 and Bcl-2 family.关键词
辛二酰苯胺异羟肟酸/结肠癌细胞/增殖/周期/凋亡/组蛋白乙酰化Key words
suberoylanilide hydroxamic acid/colon cancer cell/proliferation/cycle/apoptosis/histone acetylation分类
医药卫生引用本文复制引用
唐守龙,张晓威,陆航..辛二酰苯胺异羟肟酸对结肠癌HCT116和SW480细胞增殖、周期和凋亡的影响[J].中国肿瘤生物治疗杂志,2017,24(10):1081-1087,7.基金项目
辽宁省教育厅科学技术一般资助项目(No.LJQ2014333). Project supported by the General Foundation of Science and Technology Bureau of Education of Liaoning Provincial (No.LJQ2014333) （No.LJQ2014333）
