中国肿瘤生物治疗杂志2017，Vol.24Issue(10)：1088-1092,5.DOI:10.3872/j.issn.1007-385x.2017.10.008
ADAM17-shRNA转染的骨髓间充质干细胞对裸鼠乳腺癌移植瘤生长的影响
Effect of bone marrow mesenchymal stem cells transfected with ADAM17-shRNA on growth of implanted breast cancer in nude mouse
摘要
Abstract
Objective:To investigate the inhibitory effect of bone marrow mesenehymal stem cells (BMMSCs) transfected with ADAM 17-shRNA (adisintegrin and metalloprotease 17-shRNA) on the growth of implanted breast cancer MCF-7 cell xenograft in nude mice.Methods:BMMSCs from 3-week-old male SD rats were isolated and cultured with the whole bone marrow adherence method.BMMSCs were transfected with Lentivirus-mediated ADAM17-shRNA.Breast cancer MCF-7 cell xenograft model was successfully established in 30 nude mice after 14 days implantation of tumor cells.According to the random number table,nude mice were randomly divided into con trol group (equal volume PBS),BMMSCs group (1 × 106/ml BMMSCs) and transfection group (1 × 106/ml BMMSCs transfected with ADAM17-shRNA) with 10 nude mouses in each group.The tumor inhibition test was carried out on the 15th day by injecting BMMSCs into tail vein (0.1 ml/each,administration was carried every 3 days with a to tal of 5 times).The growth of implanted tumor was observed every day.All the nude mice were sacrificed on 16th day after treatment.The expressions of ADAM17 mRNA and ADAM17 protein in rumor tissues were detected by Real-time PCR and Westem blotting,respectively.Results:The volume of implanted tumor in control group,BMMSCs group was significantly larger than that of transfection group ([787.15 ±25.95],[767.02±28.98] vs [361.89±19.75] mm3,all P＜0.01) on D 30.The tumor inhibition rate of BMMSCs group and transfection group was significantly higher than that of control group (2.57％,53.89％ vs 0.00％,all P＜0.05).The expression of ADAM17 mRNA in control group,BMMSCs group was significantly higher than that of transfection group (1.00±0.01,0.97± 0.08 vs 0.30±0.09,P＜0.05).The expression of ADAM17 protein in control group,BMMSCs group was significantly higher than that of transfection group (0.70±0.09,0.68±0.02 vs 0.45±0.05,all P＜0.05).Conclusion:The tropism of ADAM17-shRNA to breast cancer xenograft in nude mice was accomplished by BMMSCs mediation,which may play an anti-tumor effect.关键词
解聚素-金属蛋白酶17-shRNA/乳腺癌/MCF-7细胞/骨髓间充质干细胞Key words
adisintegrin and metalloprotease 17-shRNA (ADAM17-shRNA)/breast cancer/MCF-7 cell/bone marrow mesenehymal stem cell (BMMSC)分类
医药卫生引用本文复制引用
贾文婷,蔡准,张雪鹏,张笑博,孙影,张云鹏..ADAM17-shRNA转染的骨髓间充质干细胞对裸鼠乳腺癌移植瘤生长的影响[J].中国肿瘤生物治疗杂志,2017,24(10):1088-1092,5.基金项目
唐山市科学技术研究与发展计划资助项目(No.14130256B).Project supported by Tangshan City Science and Technology Research and Development Foundation(No.14130256B) （No.14130256B）
