中华耳科学杂志2017，Vol.15Issue(3)：329-333,5.DOI:10.3969/j.issn.1672-2922.2017.03.011
MUC5AC和TNF-α在大鼠急性分泌性中耳炎模型中的表达变化
Expression of MUC5AC and TNF-αin Middle Ear Mucosa in a Rat Model of Acute Otitis Media With Effusion
摘要
Abstract
Objective To study expression of MUC5AC and TNF-αin the middle ear mucosa in rat model of acute otitis media with effusion. Methods Forty adult female Sprague-Dawlay (SD) rats were randomly divided into a control group and a experimental group. Rats in the experimental group received injection of endotoxin. The level and pattern of MUC5AC and TNF-αexpression within the middle ear epithelium were tested by qPCR and immunohistochemical as-says. Results qPCR and immunohistochemical staining results showed increased expression of MUC5AC and TNF-αin the experimental group along with time (*P<0. 05) and the MUC5AC were mainly concentrated on the goblet cells. Con-clusions Expression of MUC5AC and TNF-αis increased in rat middle ear epithelium with acute media otitis with effu-sion. Hence, it is speculated that MUC5AC and TNF-αmay participate in the process of acute media otitis with effusion.关键词
MUC5AC/TNF-α/急性分泌性中耳炎/中耳黏膜Key words
MUC5AC/TNF-α/Middle Ear Epithelium/Acute Media Otitis With Effusion分类
医药卫生引用本文复制引用
王梦航,李水静,韩琳,余力生..MUC5AC和TNF-α在大鼠急性分泌性中耳炎模型中的表达变化[J].中华耳科学杂志,2017,15(3):329-333,5.基金项目
十二五国家科技支撑计划(2012BAI12B01),国家自然科学基金科学基金项目(11202018),中国博士后科学基金项目(20110490269, 2013T60055),北京大学国际医院院内科研基金(YN2016QN03). Acknowledgements This study was supported by the Twelfth-five-year"national science and technology support plan (2012BAI12B01), the National Natural Science Foundation of China (11202018), the Post-doctoral Foundation of China (20110490269, 2013T60055) and Peking University International Hospital Research Fund (YN2016QN03). （2012BAI12B01）
