安徽中医药大学学报2017，Vol.36Issue(5)：56-61,6.DOI:10.3969/j.issn.2095-7246.2017.05.017
脑缺血再灌注大鼠海马区神经营养因子的表达及桃红四物汤的干预
Expression of Neurotrophic Factor in the Hippocampus of Rats with Cerebral Ischemia/Reperfusion and Interventional Effect of Taohong Siwu Decoction
摘要
Abstract
Objective To investigate the change in the expression of neurotrophic factor in the hippocampus of rats at different time points after cerebral ischemia/reperfusion and the interventional effect of Taohong Siwu Decoction.Methods Middle cerebral artery occlusion was performed to establish a rat model of focal cerebral ischemia/reperfusion.The experimental animals were divided into sham-operation group,model group,Taohong Siwu Decoction group,and nimodipine group.TTC staining was used to observe cerebral infarct volume,and ELISA was used to measure the expression of brain-derived neurotrophic factor (BDNF),nerve growth factor (NGF),and neurotrophin-3 (NT-3) in the hippocampus of rats.Results Compared with the sham-operation group,the model group had a significant increase in cerebral infarct volume at 12 hours,24 hours,7 days,and 14 days after surgery.Compared with the model group,the Taohong Siwu Decoction group had a significant reduction in cerebral infarct volume at 7 and 14 days after surgery,and the nimodipine group had a significant reduction in cerebral infarct volume at 12 hours,24 hours,7 days,and 14 days after surgery.Compared with the sham-operation group,the model group had significant increases in the expression of BDNF in the hippocampus at 12 hours after surgery and the expression of NGF in the hippocampus at 24 hours after surgery and had no significant changes in the expression of NT-3 at each time point.Compared with the model group,the nimodipine group and the Taohong Siwu Decoction group had a significant increase in the expression of BDNF at 7 and 14 days after surgery;the nimodipine group had a significant increase in the expression of NGF at 24 hours and 7 days after surgery;the Taohong Siwu Decoction group had no significant changes in the expression of NGF and NT-3 at each time point.Conclusion The expression of BDNF and NGF in the hippocampus of rats increases at a certain time point after cerebral ischemia,which helps with the recovery of damaged neurons.Taohong Siwu Decoction protects neurons by promoting the expression of BDNF.关键词
脑源性神经营养因子/桃红四物汤/脑缺血再灌注Key words
Brain-derived neurotrophic factor/Taohong Siwu Decoction/Cerebral ischemia/reperfusion分类
医药卫生引用本文复制引用
吴欢茹,韩岚,彭代银,季兆洁,王飞龙,汪蒙蒙,陈芳芳..脑缺血再灌注大鼠海马区神经营养因子的表达及桃红四物汤的干预[J].安徽中医药大学学报,2017,36(5):56-61,6.基金项目
国家自然科学基金项目(81473387,81503291) （81473387,81503291）
