北京林业大学学报2017，Vol.39Issue(10)：33-41,9.DOI:10.13332/j.1000-1522.20170099
NaHCO3胁迫对转TaLEA基因山新杨生长及光合、叶绿素荧光特性的影响
Effects of NaHCO3 stress on growth, photosynthesis and chlorophyll fluorescence characteristics in Populus davidiana × P. bolleana overexpressed TaLEA
摘要
Abstract
In order to find the transgenic poplar which can tolerate soda-saline soil, the lines overexpressed TaLEA gene and the control ( NT) were treated with NaHCO3 , the main components of soda-saline soil. And to evaluate salt-tolerant properties, we measured some parameters of different lines, including height and ground diameter, net photosynthetic rate ( Pn ) , intercellular CO2 concentration ( Ci ) , stomatal conductance(Gs), transpiration rate(Tr), maximal photochemical efficiency(Fv/Fm), photochemical quenching coefficient ( qP ) , non-photochemical quenching coefficient ( NPQ ) , and the actual photochemical electron yield (φPSII ) . Moreover, the accumulation of superoxide anion and hydrogen peroxide in each line was compared by staining method. The results showed that obvious changes had taken place in height and ground diameter among different lines after NaHCO3 stress. There was no significant difference before treatment, but after treating for 20 days, significant differences happened. Compared with control, all transgenic lines increased in height and ground diameter, especially the SL2 transgenic line, whose average height and ground diameter were increased by 17. 4％ and 15. 7％, respectively. Furthermore, the salt damage index of wild type was 3. 3 times of transgenic lines, and the survival rate of transgenic lines was 2. 8 times of wild type. The photosynthetic parameters and fluorescence parameters of the transgenic lines after 20 days stress were also higher than wild type, Pn and Tr were increased by 237. 38％ and 649. 02％, respectively compared with the wild type. Compared with control, Gs and Ci of transgenic lines were increased by 119. 05％ and 24. 56％ under 15 days stress, and Gs, Ci of SL2 transgenic line were increased by 150％ and 25. 81％. Besides, the average Fv/Fm of each transgenic line was 0. 68, the wild type was 0. 45. The qP and PSII of transgenic plants were only slightly higher than that of the wild type, but the NPQ of wild type was 6 times of wild type. The accumulation of superoxide anion and hydrogen peroxide in transgenic lines was less than that in wild type. All in all, transgenic lines, especially SL2, could maintain rapid growth, strong photosynthetic capacity and lower level of ROS under alkali stress environment, so SL2 was initially selected as an excellent transgenic line of poplar because of salinity tolerance.关键词
转基因/NaHCO3胁迫/生长/光合作用/叶绿素荧光/TaLEAKey words
transgene/NaHCO3 stress/growth/photosynthesis/chlorophyll fluorescence/TaLEA分类
农业科技引用本文复制引用
孙延爽,邢宝月,杨光,刘桂丰..NaHCO3胁迫对转TaLEA基因山新杨生长及光合、叶绿素荧光特性的影响[J].北京林业大学学报,2017,39(10):33-41,9.基金项目
"863"国家高技术研究发展计划项目(2011AA100201). （2011AA100201）
