动物营养学报2017，Vol.29Issue(11)：4026-4035,10.DOI:10.3969/j.issn.1006-267x.2017.11.024
蒙脱石对产蛋鸡生产性能和盲肠菌群的影响
Effects of Montmorillonite on Performance and Cecal Microflora of Laying Hens
摘要
Abstract
This study was conducted to investigate the effects of montmorillonite on performance and cecal mi-croflora of laying hens. Four hundred and eighty Brown Lohmann hens ( 75 weeks old ) were randomly as-signed to 5 groups, each of which comprised 8 replicates of 12 hens per replicate. Hens in the control group were fed a basal diet, and hens in experimental groups were fed the basal diets supplemented with 0.3, 0.6, 0.9 and 1.2 g/kg montmorillonite, respectively. The adjustment period lasted for 7 days, and the experimental period lasted for 70 days. The results showed as follows: 1) compared with the control group, the daily egg production of hens in 0.9 g/kg montmorillonite group was significantly increased ( P<0.05) , and feed to egg ratio in experimental groups tended to be higher ( P=0.073) than that in the control group during 1 to 5 weeks of the experiment. 2 ) The quantities of effective sequences and good sequences observed in 0. 3, 0. 9 and 1.2 g/kg montmorillonite groups were more than those in the control group ( P>0.05) . 3) The Beta diversity was improved in the experimental groups. The Shannon index in experimental groups tended to be higher than that in the control group ( P=0.096) , and the Simpson index tended to be lower than that in the control group ( P=0.095) . 4) At the phylum level, compared with the control group, the relative contents of Firmicutes, Proteobacteria and Spirochaetes in the experimental groups were numerically increased ( P>0.05); the relative content of WPS 2 in 0.6 g/kg montmorillonite group was significantly increased ( P<0.05) , and the Elusimi-crobia relative content in 0.3 g/kg montmorillonite group was significantly decreased compared with the control group ( P<0.05) . At the genus level, compared with the control group, the Ruminococcus relative content in the experimental groups was significantly increased ( P<0.05) , and the Parabacteroides relative content in ex-perimental groups tended to be higher than that in the control group ( P=0.067);the relative contents of Dorea and Blautia in 0.6 and 1.2 g/kg montmorillonite groups were significantly increased ( P<0.05); the Parapre-votella relative content in 0.6 g/kg montmorillonite group was significantly increased ( P<0.05) . 5) The clus-ter analysis at the genus level showed low similarity of intestinal microflora between the experimental groups and the control group. In conclusion, the bacterial diversity and species abundance of cecal microbiota of laying hens can be partially influenced by montmorillonite, and dietary supplementation with 0.9 g/kg montmorillonite tends to improve the egg production of laying hens.关键词
蒙脱石/生产性能/肠道微生物/高通量菌群/产蛋鸡Key words
montmorillonite/performance/intestinal microbiota/high-throughput sequencing/laying hens分类
农业科技引用本文复制引用
陈继发,彭灿阳,曲湘勇,季方..蒙脱石对产蛋鸡生产性能和盲肠菌群的影响[J].动物营养学报,2017,29(11):4026-4035,10.基金项目
湖南畜禽安全生产协同创新中心专项资金(CICAPS) （CICAPS）
湖南农业大学产学研合作项目(13098) （13098）
湖南省研究生科研创新项目(CX2016B299) （CX2016B299）
