动物营养学报2017，Vol.29Issue(11)：4036-4047,12.DOI:10.3969/j.issn.1006-267x.2017.11.025
饲料中添加壳寡糖和/或霉菌毒素吸附剂对凡纳滨对虾生长性能、非特异性免疫力及抗病力的影响
Effects of Dietary Chitosan Oligosaccharide and/or Mycotoxin Adsorbent on Growth Performance, Nonspecific Immunity and Disease Resistance of Litopenaeus vannamei
摘要
Abstract
An 8-week feed trial was carried out to investigate the effects of single or combined supplementation of chitosan oligosaccharide ( COS) and mycotoxin adsorbents ( MA) on the growth performance, nonspecific immunity and disease resistance of Litopenaeus vannamei. Six isonitrogenous and isolipid diets were prepared. Firstly, a basal diet was formulated as control diet ( C0 group) , and then 5 diets were formulated by adding 100 mg/kg COS ( C0. 1 group), 250 mg/kg COS ( C0. 25 group), 2500 mg/kg MA ( M2. 5 group), 100 mg/kg COS+2500 mg/kg MA ( C0. 1+M2. 5 group) , 250 mg/kg COS+2500 mg/kg MA ( C0. 25+M2.5 group), respectively. The Litopenaeus vannamei with an initial average weight of (0.23±0.02) g were randomly assigned into 6 groups with 3 replicates per group and 40 shrimps per replicate. The results showed as follows:1) the weight gain rate ( WGR) and specific growth rate ( SGR) in C0.1+M2.5 group were signifi-cantly higher than those in M2.5 groups (P<0.05), the feed conversion ratio (FCR) in C0.1+M2.5 group was significantly lower than that of other groups (P<0.05) and showed the minimum value, the maximum protein efficiency rate ( PER) was occurred in C0.1+M2.5 group and was significantly higher than that of other groups ( P<0.05) except C0.25+M0.25 group. The crude protein content of shrimp body in C0.1+M2.5 group was significantly higher than that in C0.1 group (P<0.05), and the crude lipid content of shrimp body in C0.1+M2.5 group was significantly lower than that in C0.1 group ( P<0.05) . 2) In the serum, the activity of alkaline phosphatase ( AKP) in C0.1+M2.5 and C0.25+M2.5 groups was significantly lower than that in C0.1 and C0.25 groups (P<0.05), the activities of superoxide dismutase (SOD) and phenoloxidase (PO) in C0.1+M2.5 group were significantly higher than those in M2.5 and C0.1 groups (P<0.05), and the content of malondialdehyde ( MDA) in C0.1+M2.5 group was significantly lower than that in C0.1 and C0.25 groups ( P<0.05) . In the hepatopancreas, the content of MDA in C0.1+M2.5 group was significantly lower than that in C0.1 group ( P<0.05) , and the activities of AKP, PO, SOD and lysozyme ( LZM) in C0.25+M2.5 group were lower than those in C0.1+M2.5 group, but the content of MDA was higher inversely. 3) Shrimps were challenged by Vibrio harveyi for the next 7 days, the cumulative mortality rate in C0. 1+M2.5 and C0. 25+M2.5 groups was significantly lower than that in C0 and M2.5 groups (P<0.05). It can be concluded that adding a certain amount of COS and/or MA in diets can do good to the growth, nonspecific immunity and dis-ease resistance of Litopenaeus vannamei, the combined supplementations of COS and MA show better growth, nonspecific immunity and disease resistance than single supplementation of COS and MA, and the combined supplementation of 100 mg/kg COS and 2500 mg/kg MA under this experimental condition shows the better effect.关键词
壳寡糖/霉菌毒素吸附剂/凡纳滨对虾/生长性能/非特异性免疫力/抗病力Key words
chitosan oligosaccharide/mycotoxin adsorbent/Litopenaeus vannamei/growth performance/nonspecific immunity/disease resistance分类
农业科技引用本文复制引用
黄钦成,申光荣,谭北平,章双,黑文静,董晓慧..饲料中添加壳寡糖和/或霉菌毒素吸附剂对凡纳滨对虾生长性能、非特异性免疫力及抗病力的影响[J].动物营养学报,2017,29(11):4036-4047,12.基金项目
深圳市战略性新兴产业发展专项(SWCYL20150331010019) （SWCYL20150331010019）
广东省科技计划项目(2015A020209170) （2015A020209170）
渔港建设和渔业产业发展专项(A201608C06) （A201608C06）
