动物营养学报2017，Vol.29Issue(11)：4151-4161,11.DOI:10.3969/j.issn.1006-267x.2017.11.039
海子水牛瘤胃微生物的宏基因组学分析
Metagenomic Analysis of Microorganisms in Rumen of Haizi Buffalo
摘要
Abstract
In order to investigate microorganism composition and lignocellulolytic enzymes in rumen of Haizi buffalo, rumen fluid sample of Haizi buffalo ( about 2.5 years of age, average body weight was 493 kg) was surveyed using metagenomic high-throughput sequencing. The results showed as follws: a total of 77283638 reads were obtained, which were assembled into 744712 scaffolds. And then, a total of 827044 genes were predicted by prodigal analysis. Many microorganisms involved in lignocellulose degradation were identified by gene annotation, such as Ruminococcus flavefaciens, Ruminococcus albus, Fibrobacter succinogenes and Pre-votella ruminicola. We identified potential 38011 genes encoding lignocellulolytic enzymes including glycoside hydrolase (GH) (17877 genes), glycosyl transferase (GT) (8637 genes), carbohydrate binding module (CBM) (4693 genes), carbohydrate esterase (CE) (4214 genes), polysaccharide lyase (PE) (1296 genes), and auxiliary activity (AA) (934 genes). Among GH genes, most genes belonged to GH2, GH43, GH97 and GH3 families, and a higher abundance of oligosaccharide degrading enzymes in buffalo rumen met-agenome was identified. Besides that, we also predicted that some genes could encode the cellulosome compo-nent. Compared with GH genes in the gastrointestinal metagenome of other species, the proportions of cellu-lose, hemicellulase and debranching enzyme were similar between Haizi buffalo and dairy cow. These results demonstrate that buffalo rumen is considerably enriched in functional microorganisms and enzymes involved in lignocellulose degradation with great prospects to obtain enzyme genes that may be applied in the industry.关键词
海子水牛/瘤胃/宏基因组/微生物/木质纤维素降解酶Key words
Haizi buffalo/rumen/metagenome/microorganism/lignocellulolytic enzyme分类
农业科技引用本文复制引用
张慧敏,夏海磊,黄强,绪欣,毛永江,岑宁,杨章平..海子水牛瘤胃微生物的宏基因组学分析[J].动物营养学报,2017,29(11):4151-4161,11.基金项目
国家自然科学基金青年基金(31702142) （31702142）
江苏省自然科学基金青年基金(BK20160455) （BK20160455）
江苏省高校自然科学研究面上项目资助(15KJB230004) （15KJB230004）
扬州大学大学生科技创新基金资助(x20160673) （x20160673）
