动物营养学报2017，Vol.29Issue(11)：4189-4197,9.DOI:10.3969/j.issn.1006-267x.2017.11.043
饲料中添加谷胱甘肽对黄颡鱼幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响
Effects of Dietary Glutathione on Growth Performance, Body Composition, Serum Biochemical Indices and Anti-Ammonia-Nitrogen Stress Ability of Juvenile Yellow Catfish ( Pelteobagrus fulvidraco)
摘要
Abstract
This experiment was conducted to investigate the effects of dietary glutathione on growth perform-ance, body composition, serum biochemical indices and anti-ammonia-nitrogen stress ability of juvenile yellow catfish (Pelteobagrus fulvidraco). A total of 800 juvenile yellow catfish with the mean body weight of (1.32± 0.01) g were randomly divided into 5 groups with 4 replicates per group and 40 fishes per replicate. Fish in the control group were fed a basal diet, and others in the experimental groups were fed the basal diets supplemen-ted with 100, 300, 500 and 700 mg/kg glutathione, respectively. After 56 d feeding, ammonium chloride was used to conduct an ammonia nitrogen stress test in each group. The results showed as follows:1) with the diet-ary glutathione additive amount increased, the weight gain rate, specific growth rate and protein efficiency rate increased at first but then decreased, achieving the maximum value at 300 mg/kg group, and 100 to 500 mg/kg groups were significantly higher than control group ( P<0.05);the feed conversation ratio, condi-tion factor and hepatosomatic index had no significant difference among all groups (P>0.05). 2) Compared with the control group, the whole-body crude protein content in 300 to 700 mg/kg groups and whole-body crude lipid content in 100 to 700 mg/kg groups were significantly increased ( P<0.05) . 3) Dietary glutathione had no significant effects on serum total protein, serum cholesterol and triglyceride, glucose, urea nitrogen contents and aspartate aminotransferase, alanine aminotransferase activities (P>0.05). 4) After ammonia-ni-trogen stress, the death time of juvenile yellow catfish of experimental groups was delayed than that of the con-trol group, and at 96 h, the cumulative mortality rate of juvenile yellow catfish of experimental groups was de-creased compared with the control group, and it was significantly decreased in 100 and 300 mg/kg groups ( P<0.05) . In conclusion, dietary glutathione can increase growth performance, whole-body crude protein and crude lipid contents and anti-ammonia-nitrogen stress ability. A quadratic regression analysis on specific growth rate against dietary glutathione additive amount indicated that the optimal additive amount of glutathione in the diet of juvenile yellow catfish was 357. 69 mg/kg.关键词
谷胱甘肽/黄颡鱼/生长性能/体成分/血清生化指标/氨氮应激Key words
glutathione/yellow catfish ( Pelteobagrus fulvidraco)/growth performance/body composition/serum biochemical indices/ammonia-nitrogen stress分类
农业科技引用本文复制引用
周艳玲,孙育平,曹俊明,黄燕华,王国霞,陈冰,王卫民..饲料中添加谷胱甘肽对黄颡鱼幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响[J].动物营养学报,2017,29(11):4189-4197,9.基金项目
2016年省级现代农业科技创新联盟建设项目(2016LM082,2016LM083) （2016LM082,2016LM083）
2017年海洋和渔业发展专项资金(科技攻关与研发)——"水产饲料添加剂GSH新产品研制及其护肝关键技术应用示范"项目 （科技攻关与研发）
