动物营养学报2017，Vol.29Issue(11)：4207-4215,9.DOI:10.3969/j.issn.1006-267x.2017.11.045
酵母水解物对凡纳滨对虾生长性能、肠道形态和血清非特异性免疫酶活性的影响
Effects of Yeast Hydrolysate on Growth Performance, Intestinal Morphology and Serum Nonspecific Immune Enzyme Activities of Litopenaeus vannamei
摘要
Abstract
This experiment was conducted to estimate the effects of dietary supplemental levels of yeast hydrol-ysate on growth performance, intestinal morphology and serum nonspecific immune enzyme activities of Litopenaeus vannamei. A total of 640 juvenile Litopenaeus vannamei with an initial average body weight of (0.40±0.01) g were randomly divided into four groups with four replicates and 40 Litopenaeus vannamei per replicate. Four isonitrogenous and isolipidic practical diets (42% crude protein and 8% crude lipid) were pre-pared by adding 0 ( control group) , 0. 5%, 1. 0% and 2. 0% yeast hydrolysate in a basal diet, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) there were no significant differences in survival rate ( SR) , weight gain rate ( WGR) and specific growth rate ( SGR) between experimental groups and control group (P>0.05). 1.0% supplemental group had significantly higher WGR and SGR than 2.0%supplemental group ( P<0.05) , and significantly lower feed conversion ratio ( FCR) than 2.0% supplemental group (P<0.05). Hepatopancreas somatic index (HSI) in 1.0% supplemental group was significantly higher than that in control group ( P<0.05) , but condition factor ( CF) was not significantly affected by yeast hydrol-ysate supplementation ( P>0.05) . 2) Dietary supplemental level of yeast hydrolysate had no significant influ-ences on dry matter, crude protein, crude lipid and ash contents of whole shrimp and shrimp muscle ( P>0.05). 3) Fold width, microvillus height and enterocyte height of shrimp intestine were not significantly affect-ed by dietary supplementation of yeast hydrolysate (P>0.05). 2.0% supplemental group had a significantly higher fold height in intestine than in 0.5% supplemental group ( P<0.05) . 4) Compared with control group, serum activities of phenoloxidase ( PO) and lysozyme ( LZM) of shrimp were significantly increased by dieta-ry supplementation of 1.0% yeast hydrolysate (P<0.05), and serum nitric oxidesynthase (NOS) activity reached the maximum in 1.0% supplemental group. In conclusion, dietary supplementation of 1.0% yeast hy-drolysate can significantly increase serum nonspecific immune enzyme activities of Litopenaeus vannamei, and will not exert negatively affect growth performance and intestinal morphology health of Litopenaeus vannamei.关键词
酵母水解物/凡纳滨对虾/生长性能/肠道形态/免疫酶Key words
yeast hydrolysate/Litopenaeus vannamei/growth performance/intestinal morphology/immune enzyme分类
农业科技引用本文复制引用
熊家,袁野,陆游,马红娜,周歧存,梁超,宋宏斌,邹青..酵母水解物对凡纳滨对虾生长性能、肠道形态和血清非特异性免疫酶活性的影响[J].动物营养学报,2017,29(11):4207-4215,9.基金项目
国家自然科学基金项目(41476125) （41476125）
