儿科药学杂志2017，Vol.23Issue(11)：1-5,5.DOI:10.13407/j.cnki.jpp.1672-108X.2017.11.001
基于气相色谱质谱联用技术的代谢组学在小儿原发性肾病综合征中的应用
Application of Gas Chromatography Mass Spectrometry Based Metabonomics on Primary Nephritic Syndrome in Children
王方杰 1刘韶 2王婷 1罗芳梅 1向小琴 1何周康1
作者信息
- 1. 湖南省儿童医院,湖南长沙410007
- 2. 中南大学湘雅医院,湖南长沙410008
- 折叠
摘要
Abstract
Objective:To build a network of metabolic pathways associated with primary nephritic syndrome(PNS) and to explore the biomarkers to help the diagnosis of the disease by analyzing plasma metabolites change in children with PNS.Methods:The plasma metabolites of 22 PNS children and 22 healthy children were analyzed by gas chromatography mass spectrometry (GC-MS).Preprocessing software XCMS was used to the data preprocessing,the differential metabolites between PNS children and control subjects were analyzed by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA).Results:PCA and PLS-DA model were established.The interpretation ratio and prediction ratio of variables in PCA model were 43.6％ and 12.9％ respectively,while the interpretation ratio and prediction ratio of variables in PLS-LDA model were 79.8％ and 59.2％.Fifteen kinds of metabolites were selected as the characteristic biomarkers,including cholesterol,citric acid,linoleic acid and amino acids.While the lactate,linoleic acid,hexadecanoic acid,pyruvic acid,cholesterol were increased,the others 10 kinds of metabolites were reduced obviously.Conclusion:The changes of 15 metabolites lead to the weak of glycolysis,glyconeogenesis and lipid metabolism,protein metabolism,tricarboxylic acid cycle disorders.These characteristic biomarkers provide new scientific basis for the pathogenesis of PNS and help the diagnosis and prognosis evaluation of the disease.关键词
原发性肾病综合征/气相色谱质谱联用技术/代谢组学Key words
primary nephritic syndrome/gas chromatography mass spectrometry/metabonomics分类
医药卫生引用本文复制引用
王方杰,刘韶,王婷,罗芳梅,向小琴,何周康..基于气相色谱质谱联用技术的代谢组学在小儿原发性肾病综合征中的应用[J].儿科药学杂志,2017,23(11):1-5,5.
