国际神经病学神经外科学杂志2017，Vol.44Issue(5)：501-506,6.DOI:10.16636/j.cnki.jinn.2017.05.011
大鼠癫痫持续状态后认知功能变化模式及海马脑红蛋白表达水平的实验研究
Experimental study on the changes of cognitive function and the correlation with neuroglobin expression in rats after status epilepticus
张菲菲 1程艳伟 1于敏敏 1石向群1
作者信息
- 1. 兰州军区兰州总医院神经内科,甘肃省兰州市730050
- 折叠
摘要
Abstract
Objective To observe the changes of learning and memory function after status epileptics (SE) in rats,and explore the possible mechanism.Methods Intraperitoneal injection Lithium-pilocarpine (Li-Pilo) to establish the rat model of SE.RMT-100 rats eight arm maze test was used to evaluate the learning and memory function before and after epileptic seizure in rats.Nissl staining was used to detect the neuronal damage in hippocampus.Streptavidin-biotin-peroxidase complex immunohistochemical method was used to detect the expression level of NGB.Results After SE,the hippocampal neurons were severely damaged.The expression level of NGB in CA1,CA3 and DG regions were increased,and there was a positive correlation between the number of surviving neurons in CA1,CA3 regions and the NGB expressions (r =0.206,P =0.015;r =0.306,P =0.011).With the time progress after seizure,the number of working memory error (WME) and reference memory error (RME) increased gradually.And a significant negative correlation between the number of RME and the number of surviving neurons in CA1 and CA3 area (r =-0.579,P =0.000;r =-0.454,P =0.002).There also was a significant negative correlation between the number of WME and the number of surviving neurons in hippocampal CA1 and CA3 area (r=-0.470,P=0.001;r=-0.507,P=0.000).Conclusions SE can lead to significant cognitive impairment,which may be related to the pathological changes of hippocampus induced by SE.In addition,the up-regulation of NGB expression in hippocampus after SE may be a compensatory neuroprotective mechanism of ischemic injury induced by epilepsy,and it may be helpful to improve cognitive function.关键词
癫痫持续状态/认知功能/脑红蛋白/八臂迷宫/大鼠Key words
status epilepticus/cognitive function/neuroglobin/eight arm maze/rat引用本文复制引用
张菲菲,程艳伟,于敏敏,石向群..大鼠癫痫持续状态后认知功能变化模式及海马脑红蛋白表达水平的实验研究[J].国际神经病学神经外科学杂志,2017,44(5):501-506,6.
