家畜生态学报2017，Vol.38Issue(10)：20-26,7.
长毛兔毛乳头细胞的分离培养及生长特性研究
Study on Isolation, Culture, Identification and Growth Characteristics of Dermal Papilla Cells in Angora Rabbit
摘要
Abstract
Successfully isolated and cultured rabbit dermal papilla cells (DPCs) could provide a good model for the study of rabbit hair follicle related mechanism in vitro.Skin samples were collected and separated by combination of neutral protease and collagenase D,and laid of rat tail collagen which was adherent culture matrix,separation and purification of DPCs;the morphology of DPCs cells in vitro was observed and the growth kinetics was drawn,the number of DPCs presented progressive increase in a logarithmic mode between the 4th day and the 7th day.The results of immune-fluorescence and western-blot showed that the expression of a-smooth muscle actin (α-SMA) and vimentin (VIM) were positive in DPCs;the results proved that the dermal papilla cells were hair follicle dermal source.Growth character of the subculture 3 and 9 passages DPCs was observed using ECCK-8,when DPCs entered into plateau at 120 h and 144 h,the cell density of P9 was significantly lower than that of P3 (P＜0.05);the flow cytometric analysis showed that DPCs at G0/G1 stage of P3 was significantly lower than that of P9 (P＜0.05),but G2/M stage of P3 was significantly higher than that of P9 (P＜0.05) at 72 h,96 h,122 h and 144 h.The results showed that the subculture P9 DPCs lost their properties and no longer fit functional research in vitro.This study successfully established the culturing condition of DPCs in vitro,and it had a theoretical basis for studying the regulation of hair growth and development mechanism of hair follicle in rabbit.关键词
长毛兔/毛乳头细胞/分离培养/细胞增殖/生长特性Key words
Angora rabbit/dermal papilla cells/cell isolation/cell proliferation/growth characteristics分类
农业科技引用本文复制引用
白莉雅,孙海涛,呼红梅,杨丽萍,高淑霞,姜文学..长毛兔毛乳头细胞的分离培养及生长特性研究[J].家畜生态学报,2017,38(10):20-26,7.基金项目
山东省农业科学院青年科研基金(2014QNM42) （2014QNM42）
国家自然基金青年基金(No.31501927 （）
No.31501928) （）
山东省现代农业产业技术体系特种经济动物创新团队建设项目(SDAIT-21-12) （SDAIT-21-12）
