江西农业大学学报2017，Vol.39Issue(5)：989-995,7.DOI:10.13836/j.jjau.2017127
杏鲍菇菌糠漆酶分离纯化及部分酶学性质研究
Purification and Properties of Laccase from Spent Mushroom Compost of Pleurotuseryngii
摘要
Abstract
The main aim of this study was to finda cheap raw material,spent mushroom compost of Pleuro-tuseryngii,which can be used to extract laccase.Laccase from mushroom dregsof P.eryngii was graded-precipi-tated with ammonium sulfate,frozen and dried,separated and purified with chromatography on DEAE-cellulose and its enzymatic properties were studied.The resuts showed:the laccase detected by Native SDS-PAGE was a single pure product,its molecular weight was about 44.3 ku,the purification of about 10.452-fold was achieved with an overall yield of 16.640%;the specific activity of the laccase was 6.233 U/mg,the Mc value of its oxi-dation of ABTS was 0.0870 mmol/L;the optimum temperature for laccase activity was 40℃,and the laccase could work continuously under 35℃;the optimum pH for the laccase activity was 3.8,and this laccase showed a good stability when the pH of the buffer varied from 3.8 to 4.6;the laccase activity was enhanced by Fe2+, Cu2+ and was strongly inhibited by Fe3+,Mn2+.关键词
杏鲍菇/菌糠/漆酶/分离纯化/酶学性质Key words
Pleurotuseryngii/spent mushroom compost/laccase/isolation and purification/enzymatic prop-erties分类
农业科技引用本文复制引用
刘骊,单书凯,张杰,闫苗,赖春芬,艾柳英,孙淑静..杏鲍菇菌糠漆酶分离纯化及部分酶学性质研究[J].江西农业大学学报,2017,39(5):989-995,7.基金项目
福建省自然基金杰出青年项目(2014J06010)、福建省食用菌产业重大研发平台(2014N2101)和财政部、农业部重大农技推广项目( KNJ-153011-3) Project supported by the Natural Science Foundation for Outstanding Youth Scholars of Fujian Province (2014J06010),the Major Research and Development Platform in Edible Fungus Industry of Fujian Province (2014N2101) and Ministry of Finance,Ministry of Agriculture Major Agricultural Extension Projects of China ( KNJ-153011-3) （2014J06010）
