兰州大学学报（医学版）2017，Vol.43Issue(5)：1-6,6.DOI:10.13885/j.issn.1000-2812.2017.05.001
人脐带间充质干细胞移植治疗大鼠压力性尿失禁
Transplanting human umbilical cord mesenchymal stem cells to treat stress urinary incontinence in rats
摘要
Abstract
Objective To explore the mechanism,effectiveness and feasibility of human umbilical cord mesenchymal stem cell (HUMSCs) transplantating therapy in rats with stress urinary incontinence (SUI).Methods A SUI rats model were established and SUI rats were divided into two groups,8 rats in the HUM-SCs treatment group and 8 in the experimental control group,with other normal rats in a normal control group.In the HUMSCs treatment group,HUMSCs were injected into the rats' urethra at the 4th week,after the SUI rats model was established.The leak point pressure (LPP) and maximal bladder capacity (MBC) were quantified at the first week before transplantation and the 6th week after it.The expression of desmin,myosin heavy chain (MHC) and protein gene product 9.5 (PGP9.5) in the sphincter of rat urethra were analyzed,after the rats were sacrificed.Results The values of MBC and LPP were significantly higher than those before transplantation in HUMSCs treatment group (P ＜ 0.01).In the HUMSCs treatment group,both values were significantly higher than those in the experimental control group,and the difference was statistically signifi cant (P ＜ 0.01).Immunohistochemical staining and image analysis showed that the expression of desmin,MHC and PGP9.5 in the HUMSCs group was significantly higher than that of the experimental control group (P ＜ 0.01).Conclusion HUMSCs transplantation for the treatment of SUI rats is effective and feasible.HUMSCs can improve uremic function by repairing the injured urethral sphincter and peripheral nerves.关键词
人脐带/间充质干细胞/压力性尿失禁/结蛋白/重链肌球蛋白/蛋白基因产物9.5Key words
human umbilical cord/mesenchymal stem cells/stress urinary incontinence/desmin/myosin heavy chain/protein gene product 9.5分类
医药卫生引用本文复制引用
蓝建发,潘华,罗新,石海燕..人脐带间充质干细胞移植治疗大鼠压力性尿失禁[J].兰州大学学报（医学版）,2017,43(5):1-6,6.基金项目
国家自然科学基金项目(81070459) （81070459）
