可再生能源2017,Vol.35Issue(11):1581-1587,7.
溶藻菌破碎微拟球藻细胞及其对油脂提取效果的影响研究
The effects of algicidal bacteria on cell disruption and oil extraction of Nannochloropsis sp.
摘要
Abstract
The supernatants of five strains of algicidal bacteria of Kordia sp., Bowmanella denitrificans, Aeromona hydrophila, Pseudomonas flurescens, and Bacillus cirulans were evaluated against microalgae Nannochloropsis sp. with cell disruption and oil extraction through the determination of chlorophyll a, algicidal rate and oil extraction rate. The results showed that the five strains of bacteria had good effects on the cell disruption and oil extraction. The removal rate of chlorophyll a of K.sp., A.hydrophila, B.denitrificans, P.flurescens, B.cirulans was 46.6%, 45.8%, 45.6%, 45.1%, 40.0%, respectively. And their corresponding algicidal rate was 66%, 63%, 60%, 55%, 52%, respectively. The five strains of bacteria were further evaluated for their potential to improve lipid extraction from wet algae using a mild solvent (n-hexane). The extracted oil of microalgae with K.sp., A .hydrophila, B.denitrificans, P.flurescens, B.cirulans was 0.077, 0.070, 0.066, 0.061,0.053 g/L , respectively. The oil component was further evaluated by GC, and the results showed that the supernatants had no effects on the distribution of components of Nannochloropsis sp. The results of the present study indicated that Kordia sp. was the most practical potential algicidal bacterium because of its high cell disruption rate and oil extraction rate.关键词
溶藻菌/微拟球藻/细胞破碎/油脂提取Key words
algicidal bacteria/Nannochloropsis sp./cell disruption/oil extraction分类
能源科技引用本文复制引用
邓春芳,崔岩,成家杨..溶藻菌破碎微拟球藻细胞及其对油脂提取效果的影响研究[J].可再生能源,2017,35(11):1581-1587,7.基金项目
深圳市科创委基础研究项目(JCYJ20150626110855791) (JCYJ20150626110855791)
海洋公益性行业科研专项经费(201305022). (201305022)