农业生物技术学报2017，Vol.25Issue(10)：1653-1660,8.DOI:10.3969/j.issn.1674-7968.2017.10.011
基于线粒体D-loop区四个鸡种DNA条形码和品种鉴定
DNA Barcoding and Variety Identification of Four Chicken (Gallus gallus domesticus) Breeds Based on Mitochondrial DNA D-loop Region
摘要
Abstract
China is one of the richest countries of chicken (Gallus gallus domesticus) genetic resources,with the more and more cultivated varieties appearing constantly,so how to distinguish these breeds is very important.The aim of this study was to explore the utility of mtDNA D-loop as DNA barcoding for identifying the chicken breeds.Four chicken breeds about Anka chicken,Recessive white chicken,Henan Game chicken and Silky were used as materials.By PCR amplification and sequencing technique,completed mtDNA D-loop sequences of 115 chickens were studied,and the genetic polymorphism characteristics and genetic distances between species and within population were analyzed.The results showed that,the D-loop region of four chickens were 1231～1232 bp,and the 1231 bp samples existed a base C deficiency in 859 site.In the 115 samples assay,there were 26 mutation sites and 15 haplotype.Anka chicken had 3 especial haplotypes.Recessive white chicken had 4 especial haplotypes,Henan Game chicken had 3 especial haplotypes,and Silky had 2 especial haplotypes.Haplotype diversity(Hd) and nucleotide diversity(Pi) of 4 chickens were 0.467～0.799 and 0.00 217～0.00 561 respectively.The biggest values of average number of nucleotide differences(K)(6.910),Pi(0.00561) and Hd (0.799) were all from Recessive white chicken,and the smallest value of Hd was from Silky(0.467).Kiumura 2-parameter distance and Net distance of 4 chicken breeds were 0.435％～0.940％ and 0.150％～0.712％ respectively.Clustering analysis showed that the cluster results of four chickens were consistent to the morphological class.According to the public standard of haplotypes classification,Anka chicken and Recessive white chicken mainly converged into clade E,Henan Game chicken mainly converged into clade B and Silky mainly converged into clade A.The results indicated that the divergence between 4 chicken breeds is much farther,and it was feasible to identify chicken breeds using DNA barcoding of D-loop region.This study can provide theory basis for chicken variety identification,genetic resources protection and the development and utilization.关键词
鸡/线粒体D-loop区/遗传多样性/DNA条形码/品种鉴定Key words
Chicken/Mitochondrial D-loop Region/Genetic Diversity/DNA Barcoding/Variety Identification分类
农业科技引用本文复制引用
唐修君,贾晓旭,樊艳风,高玉时,唐梦君,陈大伟,张小燕,陆俊贤,韩威..基于线粒体D-loop区四个鸡种DNA条形码和品种鉴定[J].农业生物技术学报,2017,25(10):1653-1660,8.基金项目
国家自然科学基金(No.31372277、No.31501917和No.31672382) （No.31372277、No.31501917和No.31672382）
