青岛大学医学院学报2017，Vol.53Issue(4)：386-389,4.DOI:10.13361/j.qdyxy.201704003
MMP3与Sox9基因对人椎间盘退变髓核细胞调控作用
REGULATORY EFFECT OF MMP3 AND Sox9 GENES ON HUMAN DEGENERATIVE NUCLEUS PULPOSUS CELLS
摘要
Abstract
Objective To investigate the regulatory effect of matrix metalloproteinase 3 (MMP3) gene and Sox9 gene on human degenerative nucleus pulposus ceils.Methods The lentiviral expression vectors of the MMP3 and Sox9 was constructed and transfected into human degenerative nucleus pulposus cells.According to the treatment method,the cells were divided into blank control group (group A),MMP3 silencing group (group B),Sox9 overexpression group (group C),and MMP3 silencing+Sox9 overexpression group (group D).All groups of nucleus pulposus cells were placed and cultured in a constant-temperature incubator and the medium was changed regularly.After 72 hours,qualitative real-time PCR was used to measure the mRNA expression of type Ⅱ collagen and proteoglycan in transfected human degenerative nucleus pulposus cells,and Western blot was used to mea-sure the protein expression of type Ⅱ collagen and proteoglycan in these cells.Results Groups B,C,and D had significantly higher protein and mRNA expression of proteoglycan and type Ⅱ collagen than group A (F=84.50-413.09,q=4.57-49.13,P＜0.01).There were significant differences in the protein and mRNA expression of proteoglycan and type Ⅱ collagen between group D and groups B and C (q=13.72-30.24,P＜0.01).Conclusion MMP3 silencing and Sox9 overexpression can promote the secretion of proteoglycan and type Ⅱ collagen in human degenerative nucleus pulposus cells and thus delay intervertebral disc dege-neration,and there is a synergistic effect between the two genes.关键词
基质金属蛋白酶3/性别决定相关高迁移率簇蛋白盒基因9/椎间盘退行性变/胶原Ⅱ型Key words
matrix metalloproteinase 3/Sox9/intervertebral disc degeneration/collagen type Ⅱ分类
医药卫生引用本文复制引用
贾相振,西永明,刘勇,位华卫,于涛,齐宗华..MMP3与Sox9基因对人椎间盘退变髓核细胞调控作用[J].青岛大学医学院学报,2017,53(4):386-389,4.基金项目
国家自然科学基金资助项目(81470104) （81470104）
