陕西中医2017，Vol.38Issue(11)：1617-1619,3.DOI:10.3969/j.issn.1000-7369.2017.11.061
竹沥对慢阻肺大鼠肺组织中AQP1、AQP4、AQP5、TNF-α和IL-6的影响
Effect of bamboo juice on lung tissue of rats with COPD in AQP 1 , AQP4 ,AQP5 ,TNF-and IL-6
摘要
Abstract
Objective :To observe the effect ofbamboo juice on lungtissue of rats with COPD in AQP 1 , AQP4 ,AQP5 ,TNF-and IL-6 ,To explore the possible mechanism of COPD patients with phlegm effect of juice . Methods :30 male Wistar rats ,fed after 1 weeks were randomly divided into control group ,model group and inter-vention group ,10 rats in each group ,except the blank group ,other groups were treated with cigarette smoking combined with elastaseintratracheal instillation of COPD rats model was established ,90 days later ,the intervention group in xianzhuli oral liquid gavage for 10 days ,the two other groups were saline for 10 days .After the interven-tion ,the expression of AQP1 ,AQP4 and AQP5 were observed by PCR method .The levels of TNF-and IL-6 in lung tissue were determined by enzyme linked immunosorbent assay (ELISA) .Results :Compared with the blank group ,the concentrations of AQP1 ,AQP4 and AQP5 in the lung tissue of model group and intervention group were significantly decreased (P< 0 .01) ,and the levels of TNF-and IL-6 were significantly higher (P< 0 .01) .Com-pared with the model group ,the lung tissue AQP1 and AQP5 concentrations in the intervention group were signifi-cantly increased (P< 0 .01) ,AQP4 ,TNF-and IL-6 concentrations were significantly decreased (P< 0 .01) ,the difference was statistically significant .Conclusion :Bamboo juice have a curative effect of COPD may be related to the regulation of aquaporin 1 and aquaporin 5 (AQP1) ,(AQP5) transporter ,inhibition of tumor necrosis factor al-pha (TNF-alpha) ,interleukin 6 (IL-6) expression is closely related to .关键词
肺疾病,慢性阻塞性/中医药疗法/竹沥/水通道蛋白类/动物实验/大鼠Key words
Pulmonary disease/chronic obstructive/traditional/Chinese medicine therapy/Liquor phyl-lostachys/Aquaporins/Animal experimentation/Rats分类
医药卫生引用本文复制引用
罗建江,马红霞..竹沥对慢阻肺大鼠肺组织中AQP1、AQP4、AQP5、TNF-α和IL-6的影响[J].陕西中医,2017,38(11):1617-1619,3.基金项目
新疆维吾尔自治区自然科学基金项目(2015211C149) （2015211C149）
