色谱2017，Vol.35Issue(9)：949-956,8.DOI:10.3724/SP.J.1123.2017.05001
超高效液相色谱-线性离子阱/静电场轨道阱高分辨质谱快速测定大米中15种营养成分
Rapid determination of 15 nutrients in rice by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry
摘要
Abstract
A rapid method was developed for the simultaneous determination of 15 nutrients containing eight vitamin E, six γ-oryzanols and β-carotene in rice by ultra high performance liq-uid chromatography-linear ion trap/orbitrap high resolution mass spectrometry ( UHPLC-LTQ/Orbitrap HRMS) . The analytes were extracted by methanol containing 0. 05% ( v/v) 2,6-di-tert-butyl-4-methylphenol ( BHT) under the ultrasonic condition, then separated by a Poroshell 120 PFP column (150 mm×3. 0 mm, 2. 7 μm) with a gradient elution program using 0. 1% ( v/v) formic acid aqueous solution and methanol containing 0. 1% ( v/v) formic acid as mobile pha-ses. The detection was performed using an LTQ/Orbitrap HRMS detector with full scan in posi-tive ion mode. Fifteen nutrients were simultaneously separated within 13 min. Furthermore, matrix effects of the white and brown rices were investigated. The correlation coefficients ( r) were≥0. 9950 in the linear ranges of the 15 nutrients. The limits of detection ( LODs, S/N=3) and limits of quantification ( LOQs, S/N=10) of the 15 nutrients were 0. 2-1. 8 μg/L and 0. 7-6. 1 μg/L, respectively. At the three spiked levels, the recoveries were 73. 2%-101. 5% with the relative standard deviations ( RSDs ) ranging from 1. 1% to 5. 0% ( n = 3 ) . The method is accurate, efficient and reliable. It is suitable for the simultaneous determination of various nutrients in rice.关键词
超高效液相色谱/线性离子阱/静电场轨道阱高分辨质谱/营养成分/维生素E/γ-谷维素/β-胡萝卜素/大米Key words
ultra high performance liquid chromatography ( UHPLC)/linear ion trap-orbitrap high resolution mass spectrometer ( LTQ/Orbitrap HRMS )/nutrients/vitamin E/γ-oryzanol/β-carotene/rice分类
化学化工引用本文复制引用
朱丽,谭微,彭祖茂,邓梦雅,张协光,杨国武..超高效液相色谱-线性离子阱/静电场轨道阱高分辨质谱快速测定大米中15种营养成分[J].色谱,2017,35(9):949-956,8.基金项目
广东省质量技术监督局科技项目(2017CZ12).Administration of Quality and Technology Supervision of Guangdong Province Project (No.2017CZ12) . （2017CZ12）
