色谱2017，Vol.35Issue(9)：963-969,7.DOI:10.3724/SP.J.1123.2017.06005
基质分散固相萃取-高效液相色谱-串联质谱法检测红薯中的植物生长调节剂
Determination of the plant growth regulators in batatas by matrix solid-phase dispersive extraction and high performance liquid chromatography with tandem mass spectrometry
摘要
Abstract
An analytical method based on matrix solid-phase dispersive ( MSPD) extraction and high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS ) was established for the determination of forchlorfenuron, 6-benzylaminopurine ( 6-BA) , N-( 2-ethyl-hexyl)-5-norbornene-2,3-dicarboximide ( MGK 264) and paclobutrazol in batatas. The samples were dispersed with silica by grinding, and then extracted with methanol. The determination was carried out on a Thermo hypersil GOLD C18 column (150 mm×2. 1 mm, 5μm) in gradient elution with mobile phases of methanol and ammonium formate ( 5 mmol/L, containing 0. 1%( v/v) formic acid) and detected with tandem mass spectrometry using selected reaction moni-toring ( SRM) mode. The quantification was performed using external standard calibration, and the calibration curves were performed in the ranges of 10. 8-216. 0 ng/g ( forchlorfenuron ) , 10. 8-216. 0 ng/g ( 6-BA ) , 12. 5-250. 0 ng/g ( MGK 264 ) , 10. 2-204. 0 ng/g ( paclobutrazol ) with the correlation coefficients larger than 0. 96. The limits of quantification ( LOQs) were 0. 1-0. 3 ng/g. The spiked recoveries at the levels of 50, 100 and 200 ng/g of these four plant growth regulators were in the range of 85. 3%-116. 0%, and the relative standard deviations were 0. 6%-22. 7%. This method has advantages of simple operation and good accuracy, and can be used for the quantitative determination of forchlorfenuron, 6-BA, MGK 264 and paclobutrazol in batatas.关键词
基质分散固相萃取/高效液相色谱-串联质谱/氯吡脲/6-苄氨基嘌呤/增效胺/多效唑/红薯Key words
matrix solid-phase dispersive ( MSPD)/high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS )/forchlorfenuron/6-benzylaminopurine ( 6-BA )/N-(2-ethylhexyl)-5-norbornene-2/3-dicarboximide (MGK 264)/paclobutrazol/batata分类
化学化工引用本文复制引用
张婧,万慧慧,张华..基质分散固相萃取-高效液相色谱-串联质谱法检测红薯中的植物生长调节剂[J].色谱,2017,35(9):963-969,7.基金项目
国家自然科学基金项目(21505014) （21505014）
大连理工大学引进人才科研专题项目(DUT15RC(4)13).National Natural Science Foundation of China (No.21505014) （DUT15RC(4）
Dalian University of Technology for Talent Introduction (No.DUT15RC(4)13). （No.DUT15RC(4）
