色谱2017，Vol.35Issue(11)：1192-1197,6.DOI:10.3724/SP.J.1123.2017.08002
半制备型液相色谱法分离纯化茶叶鲜叶中7种儿茶素类化合物
Isolation and purification of seven catechin compounds from fresh tea leaves by semi-preparative liquid chromatography
摘要
Abstract
An effective and simple method was established to simultaneously purify seven tea catechins ( gallocatechin ( GC) , epigallocatechin ( EGC) , catechin ( C) , epigallocatechin-3-O-gallate ( EGCG ) , epicatechin ( EC ) , epigallocatechin-3-O-( 3-O-methyl )-gallate ( EGCG3"Me ) and epicatechin-3-O-gallate ( ECG ) ) from fresh tea leaves by semi-preparative high perform-ance liquid chromatography ( HPLC) . Fresh leaves of Tieguanyin tea were successively extrac-ted with methanol and chloroform. Then crude catechins were precipitated from the aqueous fraction of chloroform extraction by adding lead subacetate. Crude catechins were used for the isolation of the seven target catechin compounds by semi-preparative HPLC. Methanol-water and acetonitrile-water were sequentially used as mobile phases. After two rounds of semi-preparative HPLC, all target compounds were achieved with high purities (>90%) . The pro-posed method was tested on two additional tea cultivars and showed similar results. This method demonstrated a simple and efficient strategy based on solvent extraction, ion precipitation and semi-preparative HPLC for the preparation of multiple catechins from tea leaves.关键词
半制备色谱/离子沉淀/分离纯化/儿茶素类化合物/茶叶Key words
semi-preparative high performance liquid chromatography ( HPLC)/ion precipita-tion/isolation and purification/catechin compounds/tea leaves分类
化学化工引用本文复制引用
龚智宏,陈思,高江涛,李梅红,汪厦霞,林军,俞晓敏..半制备型液相色谱法分离纯化茶叶鲜叶中7种儿茶素类化合物[J].色谱,2017,35(11):1192-1197,6.基金项目
福建省自然科学基金(2016J01108). Fujian Natural Science Foundation of China ( No. 2016J01108) . （2016J01108）
