现代检验医学杂志2017，Vol.32Issue(5)：1-3,3.DOI:10.3969/j.issn.1671-7414.2017.05.001
PD-L1在原发性胆汁性肝硬化患者外周血单个核细胞中的变化及意义
Change of PD-L1 in Peripheral Blood Mononuclear Cells of Patients with Primary Biliary Cirrhosis and Its Significance
摘要
Abstract
Objective To explore the expression of PD-L1 in peripheral blood mononuclear cells (PBMC) from patients with primary biliary cirrhosis (PBC) and its significance.Methods Peripheral blood of 23 healthy individuals and 23 patients with PBC diagnosed in Changhai Hospital was collected during February 2013 to December 2015.PBMC was separated by density gradient centrifugation,then cultured.The mRNA expression of PD-L1 in PBMC was examined by real-time fluorescent quantitative PCR (qRT-PCR),the expression of cytokines such as TNF-α,TGF-β,IL-23,IL-17 in medium was examined by enzyme-linked immunosorbent assay (ELISA).Two independent samples' t test was used to compare variables in two groups.Pearson correlative coefficient was used to reveal the relationship between two variables.Results mRNA expression of PD-L1 in experiment and control groups was 0.23±0.08 vs 1.27±0.40 (t=12.23,P＜0.000 1) and there was statistical significance.Pearson correlation analysis revealed that PD-L1 was negatively correlated with IL-23(r=-0.531,P =0.009) with difference statistically.Conclusion PD-L1 might involve in the development of PBC,and could be the potential biomarker for predicting and treating this disease.关键词
原发性胆汁性肝硬化/程序性细胞死亡配体1/肿瘤坏死因子-α/白细胞介素-23Key words
primary biliary cirrhosis/PD-L1/TNF-α/IL-23分类
医药卫生引用本文复制引用
李腾达,钱琤,邓安梅,陈燕,谷明莉,黄元兰,张薇薇,孙懿,郭杰,刘云,徐贵霞..PD-L1在原发性胆汁性肝硬化患者外周血单个核细胞中的变化及意义[J].现代检验医学杂志,2017,32(5):1-3,3.基金项目
973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019). （2013CB531606）
