现代检验医学杂志2017，Vol.32Issue(5)：13-15,117,4.DOI:10.3969/j.issn.1671-7414.2017.05.004
microRNA-125b在葛根素诱导人卵巢癌细胞SKOV3凋亡中的作用研究
Role of MicroRNA-125b in Human Ovarian Cancer Cell SKOV3 Induced Apoptosis by Puerarin
摘要
Abstract
Objective To explore whether puerarin induced apoptosis of SKOV3 ovarian cancer cells by microRNA-125b.Methods Using the qRT-PCR technique to detect the change of microRNA-125b after puerarin pretreated SKOV3.Using RNA interference technology to inhibit microRNA-125b expression in SKOV3 cells.Using Western blot technique to detect apoptosis related proteins after microRNA-125b lower expression.Results Puerarin could significantly inhibit ovarian cancer cell SKOV3 proliferation activity and promote its apoptosis related proteins expression.And puerarin can promote the expression of microRNA-125b.Inhibition of microRNA-125b expression in ovarian cancer cell SKOV3 could reduce apoptosis protein expression in SKOV3 cell.Conclusion MicroRNA-125b was involved in puerarin induced SKOV3 cell apoptosis,and prompt microRNA-125b is key molecular of the drug resistance in SKOV3.关键词
卵巢癌/葛根素/microRNA-125b/凋亡Key words
ovarian/puerarin/microRNA-125b/apoptosis分类
医药卫生引用本文复制引用
张悦,段海霞,暴蕾,符乔珊..microRNA-125b在葛根素诱导人卵巢癌细胞SKOV3凋亡中的作用研究[J].现代检验医学杂志,2017,32(5):13-15,117,4.基金项目
国家自然科学基金青年项目:Notch信号通路介导的神经干细胞自噬在铅神经毒性中的重要作用(81602815). （81602815）
