新疆农业科学2017，Vol.54Issue(8)：1414-1421,8.DOI:10.6048/j.issn.1001-4330.2017.08.005
干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响
Influences of Brassinolide under Drought Stress on the Expressions of NAC Transcription Factors in Germination Stage of Cotton Seeds
摘要
Abstract
[Objective] In order to investigate the expression of NAC transcription factors during seed germination of cotton(Gossypium hirsutum) under drought stress and to analyze the function of NAC transcription factors in the pathway of brassinolide (BR) regulation for drought resistance in cotton.[Method] The gene sequences of upland cotton NAC transcription factor family were used to amplify the 6 genes of GhNAC from cotton Xinluzaol7.Quantitative RT-PCR was used to detect the change of NAC gene expression in cotyledon and radicle of cotton seeds at germination stage after seed soaked with different concentrations of brassinolide under drought stress.[Result] In cotton cotyledons,the expressions of GhNAC1 under drought stress were suppressed by different concentrations of brassinolide,the expressions of GhNAC2 and GhNAC3 were induced by 0.25 mg/L brassinolide.The expressions of GhNAC5 were only induced by 0.5 mg/L brassinolide,and the expressions of GhNAC4 and GhNAC6 did not change significantly.However,in cotton radicle,the expressions of GhNAC1 were also inhibited by different concentrations of brassinolide,the expressions of GhNAC2 and GhNAC3 were induced by 0.25 mg/L brassinolide.At same time,high expressions of GhNAC3 were induced by 2 mg/L brassinolide,the expressions of GhNAC5 did not change significantly,the expressions of GhNAC4 and GhNAC6 were induced by the high concentration of brassinosteroids.[Condusion] The expressions of GhNAC1 in cotton cotyledons and radicles during seed germination were inhibited by brassinolide,and the expressions of GhNAC2 and GhNAC3 was induced by low concentration of brassinolide.The results showed that brassinolide could affect the expression of GhNAC in response to drought stress.关键词
棉花/种子萌发/干旱胁迫/油菜素内酯/NAC转录因子/基因表达Key words
short ultivars/growth stages/major gene plus polygene inheritance/genetic analysis分类
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严晓红,包秋娟,张富春..干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响[J].新疆农业科学,2017,54(8):1414-1421,8.基金项目
国家自然科学基金-新疆联合基金重点项目“棉花水分高效利用的关键基因的挖掘及抗旱种质材料的创制”(U1303282)Joint key fund of National Natural Science Foundation of China and Xinjiang "Exploring key genes related to high-efficiency water utilizationand creating of drought resistant materials in cotton" (U1303282) （U1303282）
