新疆农业科学2017，Vol.54Issue(8)：1475-1480,6.DOI:10.6048/j.issn.1001-4330.2017.08.013
新疆红枣软腐病病原菌的鉴定
Identification of the Pathogen of Jujube Soft Rot in Xinjiang
摘要
Abstract
[Objective] The pathogen of soft rot disease of jujube was accurately identified so as to lay a foundation for disease prevention and control.[Method] The suspected pathogens were obtained by the conventional isolation technology and were preliminarily identified by morphological features and loop validation.The fragments of ITS gene of the suspected pathogens were obtained by the PCR technique.When the related trains of Rhizopus oryzae in GenBank were compared and analyzed,the genetic evolutionary tree was constructed and the pathogen was identified by morphological identification and pathogenicity experiments.[Result]According to the morphological characteristics and the pathogenicity test results,the morphological characteristics of Rhizopus soft rot pathogen of Xinjiang jujube were similar to that of Rhizopus oryzae.The phylogenetic analysis of the pathogen showed that the ITS gene of pathogen of jujube soft rot showed 99 ％ to 100 ％ similarity with part ITS gene of Rhizopus oryzae in GenBank,and in the analysis of phylogenetic tree,they were clustered in the same group.[Conclusion] Based on the morphological characteristics,pathogenicity test and molecular identification of the pathogen,the pathogen of jujube soft rot was identified as Rhizopus oryzae.关键词
枣软腐病/rDNA-ITS/病原鉴定/遗传进化树分析Key words
jujube soft rot/rDNA-ITS/pathogens identification/phylogenetic analysis分类
农业科技引用本文复制引用
白剑宇,宋峰惠,吴正保,刘正兴,崔燕华,史彦江..新疆红枣软腐病病原菌的鉴定[J].新疆农业科学,2017,54(8):1475-1480,6.基金项目
中国博士后科学基金面上项目“新疆红枣黑斑病菌的田间追踪检测技术研究”(2014M552565XD) （2014M552565XD）
新疆维吾尔自治区林业发展补助资金项目“南疆红枣主产区红枣主要病害普查鉴定及田间追踪监测技术研究”(XJLKY2016056)China Postdoctoral Science Foundation Project "The Tracking and Detection Technology of Pathogen of the Black Spot of Jujube in Fields in Xinjiang" (2014M552565XD) and Xinjiang Uygur Autonomous Region Research Institute Fund Project "Study on the Monitoring Technology of Screening and Identification of Main Diseases and Field Tracking in the Main Jujube Producing Areas in Southern Xinjiang"(KYGY2016055) （XJLKY2016056）
