新疆农业科学2017，Vol.54Issue(9)：1613-1620,8.DOI:10.6048/j.issn.1001-4330.2017.09.006
盐角草PsaH基因的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of Salt-tolerance of PsaH Gene from Salicornia europaea
摘要
Abstract
[Objective] To clone a novel PsaH gene from Salicornia europaea,and analyze its biological information for better understanding its role in function of salt-tolerance.[Method] Salicornia europaea was used as the plant material to done the full-length cDNA sequence of SePsaH by RT-PCR.The encoding region and amino acid sequence of PsaH gene,and the structure and function of protein encoded by protein were analyzed by NCBI,MEGA and Expasy and other online Bioinformatics bioinformatics software for SePsaH gene.[Result] Full-length cDNA sequence encoding photosystem Ⅰ reaction center subunit H was cloned from Salicornia europaea and designated by the name of SePsaH,which was an opening reading frame of 438bp encoding 145 amino acids.The putative protein molecular weight was 15.3kD and its theoretical isoelectric points was 9.84,SePsaH was a hydrophilic protein;Phylogenetic analysis showed that SePsaH gene and Spinacia oleracea were closely related;Through the conservative analysis of the protein,we found that there were 4 conserved domain structures.[Conclusion] SePsaH gene was cloned,which has laid the foundation for further study on the gene function and the role in salt-tolerance of Salicornia europaea.关键词
盐角草/SePsaH基因/生物信息Key words
Salicornia europaea/photosystem Ⅰ reaction center subunit H/bioinformatics分类
农业科技引用本文复制引用
郝晓燕,李建平,足木热木·吐尔逊,高升旗,黄全生..盐角草PsaH基因的克隆及生物信息学分析[J].新疆农业科学,2017,54(9):1613-1620,8.基金项目
新疆维吾尔自治区自然科学基金项目“碱蓬SsPSI基因提高转基因烟草耐旱性的研究”(2015211A028) （2015211A028）
新疆农业科学院优秀青年科技人才基金“盐生植物盐角草耐盐基因的发掘与功能研究”(xjnky-2012-003) （xjnky-2012-003）
Natural Science Foundation of Xinjiang " Study on Improving Drought Resistance of Transgenic Tobacco by Suaeda Salsa SsPSI Gene" (2015211A028) and Outstanding Young Scientific Talent Foundation of Xinjaing Academy of Agricultural Sciences "Discovery and Function of Salt Tolerant Genes in Halophytes" (xjnky-2012-003) （2015211A028）
