新疆农业科学2017，Vol.54Issue(9)：1621-1626,6.DOI:10.6048/j.issn.1001-4330.2017.09.007
利用SRAP标记快速检测西瓜杂交种子纯度
Using SRAP Markers for Rapid Detection of Zaojia (84-24) Watermelon Hybrid Seed Purity
摘要
Abstract
[Objective] To screen out one SRAP marker to detect seed purity,the watermelon hybrids of Zaojia (84-24) and its paternal and female lines were used to study the polymorphorrism of amplifiable spectral bands.[Method] Polymorphic primer pairs were selected from 28 SRAP primer pairs for Zaojia (84-24)hybrids and their parents in this experiment.The SRAP primers with good polymorphism,clear bands and stable primers were selected as candidate markers,and the purity of the 100 hybrid seeds was tested and verified.[Result] The results showed that 28 pairs of primers had 22 pairs of primers,and polymorphic bands were amplified between Ft and its parents.The ratio of polymorphism was 78.6％,among which,there was only one pair of complementary type SRAP primer(Me6F/ Em2R)with male display and lack of female parent,The result showed the purity of hybrid seeds was 99.0％,which was basically the same as the field morphological identification (99.1％).[Conclusion] SRAP primer (Me6F/Em2R),in combination with Polyacrylamide gel electrophoresis can be used to detect the purity of Zaojia (84-24) hybrid seed rapidly and accurately.There is a great prospect of application in using this method.关键词
西瓜/SRAP标记/杂交种子纯度/快速检测Key words
watermelon/SRAP molecular marker/hybrid seed purity/rapid detection分类
农业科技引用本文复制引用
韩宏伟,王浩,佘建华,王强,庄红梅,刘会芳,李宁..利用SRAP标记快速检测西瓜杂交种子纯度[J].新疆农业科学,2017,54(9):1621-1626,6.基金项目
新疆维吾尔自治区公益性科研院所基本科研业务费专项“蔬菜品种收集引进及筛选利用研究”(KYGY2016119) （KYGY2016119）
新疆农业科学院青年基金项目“基于SRAP标记的西瓜杂交种早佳(84-24)纯度的快速检测体系构建”(xjnkq-2015008) （84-24）
The Basic Research Funding for the Public Welfare Research Institutes of Xinjiang Uygur Autonomous Region "Study on Collection,Introduction and Selection of Vegetable Germplasm" (KYGY2016119) （KYGY2016119）
The Youth Fund Project of Xinjiang Academy of Agricultural Sciences "The Construction of Rapid Detection System to Detect the Purity of Zaojia 84-24 Watermelon Seed by SRAP Markers (xjnkq-2015008) （xjnkq-2015008）
