新疆农业科学2017，Vol.54Issue(9)：1713-1720,8.DOI:10.6048/j.issn.1001-4330.2017.09.018
FeCl3溶液引起葡萄白藜芦醇积累与Halliwell-Asada途径的偶联关系
Coupling Mechanism between-Resveratrol Accumulation Induced by FeCl3 Solution and Halliwell-Asada Pathway in Grapevine
摘要
Abstract
[Objective] FeCl3 solution causes the accumulation of resveratrol,but the mechanism is not clear.Here we aim to study the relationship between the Halliwell-Asada (H-A) pathway and resveratrol accumulation from the perspective of damage protection.[Method] After FeCl3 solution treatment of grape leaves,the degree of cell damage and the level of reactive oxygen species were detected,and the expression of resveratrol and H-A pathway related genes were analyzed.[Result] The results showed that the oxidative stress level of leaves treated with FeCl3 solution were positively correlated with the damage of foliar tissue.A significant resveratrol accumulation was also observed.In addition,the maximum content of resveratrol reached 36.83 μg/g after treatment in a dose-dependant manner.The gene expression levels of resveratrol metabolic enzymes and key enzymes in H-A pathway were increased in FeCl3 treatment,such as PAL,C4H,4CL,STS,APX and MDAR except DHAR.Resveratrol contents in H2O2 co-treatment were raised,contrary to co-treatments of antioxidant reagents NAC and GSH-EE.[Conclusion] FeCl3 solution decreased ROS scavenging capability of H-A pathway,thus causing oxidative stress and leading to resveratrol accumulation in grapevine samples,which underlines a correlation between resveratrol metabolism and H-A pathway.关键词
白藜芦醇/细胞损伤/H-A途径/ROS积累/美国红地球葡萄Key words
resveratrol/foliar injury/H-A pathway/ROS accumulation/Vitis vinifera L.cv.Red Globe分类
农业科技引用本文复制引用
董锦蕾,李月荣,王晓琴,张波..FeCl3溶液引起葡萄白藜芦醇积累与Halliwell-Asada途径的偶联关系[J].新疆农业科学,2017,54(9):1713-1720,8.基金项目
国家自然科学基金项目“葡萄芪类物质积累与氧应激反应偶联机制研究”(31160058),新疆兵团重点领域科技攻关项目“葡萄废弃物中芪多酚的富集及抗肿瘤研究”(2014BA029) （31160058）
NSFC "Study on the reaction mechanism of coupling stilbene accumulation and oxidative stress in grape" (31160058),science and technology research projects in key fields of XPCC " Enrichment along with anti-tumor study of stilbene polyphenols in grape waste" (2014BA029) （31160058）
