眼科新进展2017，Vol.37Issue(11)：1010-1014,5.DOI:10.13389/j.cnki.rao.2017.0256
血管内皮生长因子靶向抗体对大鼠角膜碱烧伤后新生血管抑制作用的研究
Inhibitory effects of anti-vascular endothelial growth factor (VEGF) antibody MIL60 on alkali-induced corneal angiogenesis in rats
摘要
Abstract
Objective To investigate the efficacy of an anti-vascular endothelial growth factor (VEGF) antibody,MIL60,in inhibiting corneal neovascularization (CoNV) formation in a rat model of alkali cauterization and its involved mechanisms.Methods Rat CoNV model induced by alkali burn was founded in the right eyes,and then 72 cases were randomly divided into four groups according to the subconjunctival administration of medicine next day after the successful establishment of this model:25mg· mL-1 MIL60 group,dexamethasone group,MIL60 solvent group and NaCl group.Then CoNV was observed for recording the its length and the involved area using digital photograph.Next the rats were sacrificed on day 7,14,21 and 28,followed by the collection of rats' cornea for HE and immunohistochemical staining to analyze the protein expression of VEGF,VEGF receptor-1 (VEGFR-1),VEGFR-2 and matrix metallopeptidase-9 (MMP-9).Results At each time point,the area and length of CoNV in the 25 mg· mL-1 MIL60 and dexamethasone group were significantly less than those in the MIL60 solvent and NaC1 group,and the differences were statistically significant (all P ＜0.01),and 25 mg· mL-1 MIL60 group had the similar CoNV area and length with the dexamethasone group (all P ＞ 0.05).Moreover,HE and immunohistochemical staining showed that MIL60 could inhibit the protein expression of VEGF,VEGFR-1,VEGFR-2 and MMP-9,which could explain its effective anti-angiogenic activity.Conclusion Subconjunctival administration of MIL60 can significantly inhibit corneal neovascularization formation and alleviate the inflammation in rats suffered from alkali burn.关键词
血管内皮生长因子/MIL60/角膜新生血管/碱烧伤Key words
vascular endothelial growth factor/MIL60/corneal neovascularization/alkali cauterization分类
医药卫生引用本文复制引用
王群,姜严明,赵杰,侯宝杰,吕明,黄一飞..血管内皮生长因子靶向抗体对大鼠角膜碱烧伤后新生血管抑制作用的研究[J].眼科新进展,2017,37(11):1010-1014,5.基金项目
武警总医院院内课题(编号:wz2015011)Intrahospital Project of General Hospital of CAPF (No:wz2015011) （编号:wz2015011）
