中国动脉硬化杂志2017，Vol.25Issue(10)：978-984,7.
ox-LDL经由LOX-1受体对PPARγ-LXRα-ABCA1通路激活的作用研究
Ox-LDL activate PPARγ-LXRα-ABCA1 to transcript by LOX-1 receptor
摘要
Abstract
Aim To investigate the effect of oxidized low density lipoprotein (ox-LDL) on activation of PPARγ-LXRoα-ABCA1 pathway via lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) receptor.Methods The expression of liver X receptor cα (LXRa) and ABCA 1 was detected by stimulating J774A.1 macrophages with concentration gradient ox-LDL (0 ～ 40 mg/L,12 h),the expression of LOX-1 was detected by laser confocal microscopy and Western blot,the transcriptional activation of peroxisome proliferators-activated receptor γ (PPAR γ) was detected by double luciferase reporter gene,the expression of LXRα and ATP-binding cassette transporter A1 (ABCA1) protein was detected after incubation with J774A.1 macrophages by LOX-1 siRNA and PPARγ siRNA silencing,ox-LDL (30 mg/L,12 h).Results Ox-LDL significantly up-regulated the expression of LXRα and ABCA1 in J774A.1 cells (significantly different from the cell untreated by ox-LDL as control,P＜0.01,n =3).LOX-1 overexpression of PPARγ double luciferase reporter gene system showed that ox-LDL increased the transcriptional activity of PPARγ by LOX-1.LOX-1 siRNA and PPARγ siRNA were treated with J774A.1 macrophages,respectively,and LXRoα and ABCA1 were expressed (significantly different from the cell untreated by siRNA as control,P＜0.01,n =3).Conclusion Ox-LDL activates PPARγ transcriptional activity by LOX-1 receptor,thereby upregnlating LXRoα and ABCA1 protein expression and activating PPARγ-LXRα-ABCA1 signal pathway.关键词
血凝素样氧化型低密度脂蛋白受体1/氧化型低密度脂蛋白/过氧化体增殖物激活型受体γ/肝X受体α/三磷酸腺苷结合盒转运体A1Key words
Lectin-like oxidized low density lipoprotein receptor-l/Oxidized low density lipoprotein/Peroxisome proliferators-activated receptor γ/Liver X receptor α/ATP-binding cassette transporter A1分类
生物科学引用本文复制引用
余承洁,李敬达,修志龙,刘庆平..ox-LDL经由LOX-1受体对PPARγ-LXRα-ABCA1通路激活的作用研究[J].中国动脉硬化杂志,2017,25(10):978-984,7.基金项目
国家自然科学基金项目(81270361) （81270361）
