中国动脉硬化杂志2017，Vol.25Issue(10)：997-1001,5.
高表达microRNA-22对缺氧心肌细胞的保护作用及机制
Over-expression of microRNA-22 protects cardiomyocytes from ischemic injury
摘要
Abstract
Aim To investigate the protection effect of microRNA-22 on ischemic cardiomyocyte.Methods Cardiomyocytes were transfected with microRNA-22 and cell activities were assessed.MTT was used to assess cell activities,EdU to assess DNA proliferation and Caspase-3 to assess cell apoptosis.Results MicroRNA-22 was transducted successfully using adeno-virus.The transduction efficiency reached 95％.Over-expression of microRNA-22 down-regulated PTEN level.Over-expression of microRNA-22 increased cell activities,DNA proliferation and decreased cell apoptosis.Conclusion Over-expression of microRNA-22 protected cardiomyocytes from ischemic injury.关键词
microRNA-22/心肌细胞/细胞特性Key words
MicroRNA-22/Cardiomyocyte/Cell activities分类
医药卫生引用本文复制引用
凌琳,凌子成,顾少华..高表达microRNA-22对缺氧心肌细胞的保护作用及机制[J].中国动脉硬化杂志,2017,25(10):997-1001,5.基金项目
江苏省自然科学青年基金项目(BK20140296) （BK20140296）
苏州市科教兴卫项目(KJXW2013004) （KJXW2013004）
苏州大学校级青年教师自然科学基金项目(SDY2013A29) （SDY2013A29）
