中国动脉硬化杂志2017，Vol.25Issue(10)：1002-1007,6.
曲美他嗪对缺氧诱导原代大鼠心肌细胞凋亡的影响
The influence of trimetazidine on the apoptosis induced by hypoxia in the rat cardiac myocytes
付尧 1汉雯 1白小涓1
作者信息
- 1. 中国医科大学附属盛京医院老年病科,辽宁省沈阳市110001
- 折叠
摘要
Abstract
Aim To study the protective effect of trimetazidine (TMZ) on hypoxia jury in the rat cardiac myocytes.Methods Primary myocardial cells of rat were cultured in incubator at 37C with 94％ N2 + 5％ CO2 + 1％ O2.Cell viability was detected by MTT.Apoptosis was detected by Hoechst staining.Mitochondrial membrane potential (MMP) was detected by TMRE.Mitochondrial energy metabolism was detected by Oxygraph-2k.Caspase-3,cytochrome C and respiratory chain complex enzymes were detected by Western blot.Results Hypoxia could induce apoptosis in the rat cardiac myocytes,decrease of MMP,cytochrome C release to cytosol.Furthermore,hypoxia could decrease state 3 respiration and respiratory control rate,increase state 4 respiration and decrease the expression of respiratory chain complex enzymes.Meanwhile,TMZ could play a protective effect against the apoptosis induced by hypoxia in the rat cardiac myocytes.It may be related to its protective role on MMP and respiratory chain complex enzymes.Conclusion TMZ could inhibit the apoptosis of rat cardiac myocytes induced by hypoxia via the mitochondrial pathway.关键词
缺氧/曲美他嗪/心肌细胞/细胞凋亡/线粒体能量代谢Key words
Hypoxia/Trimetazidine/Cardiac myocytes/Cell apoptosis/Mitochondrial energy metabolism分类
医药卫生引用本文复制引用
付尧,汉雯,白小涓..曲美他嗪对缺氧诱导原代大鼠心肌细胞凋亡的影响[J].中国动脉硬化杂志,2017,25(10):1002-1007,6.
