邓文浩 1李树仁 1张跃华 2郝潇 1李沙 1马玉龙1
作者信息
- 1. 河北医科大学研究生学院,河北省石家庄市050011
- 2. 河北省人民医院心脏一科,河北省石家庄市050051
- 折叠
摘要
Abstract
Aim To investigate the role of galectin-3 (Gal-3) in ventricular remodeling of heart insufficiency,via the pathway of Gal-3 specific competitive antagonist--modified citrus pectin (MCP) inhibiting Gal-3.Methods 30 rabbits were randomly divided into sham operation group,cardiac insufficiency group and MCP group.A model of cardiac dysfunction after myocardial infarction was made by ligating the anterior descending branch of coronary artery.After the model was successfully made,MCP group was treated with 75 g/L MCP allocated with normal saline,and intragastric administration for 4 weeks with 2 mL/(kg · d),and sham operation group and cardiac insufficiency group received the intragastric administration of 2 mL/(kg · d) normal saline for 4 weeks.Before operation and 2,4,6 weeks after operation,cardiac function was measured by cardiac ultrasound,and serum levels of Gal-3 and brain natriuretic peptide (BNP) were detected by ELISA.The mRNA expressions of Gal-3,collagen Ⅰ and Ⅲ in myocardial tissue were detected by real-time quantitative PCR,and the ratio of Ⅰ / Ⅲ was calculated.6 weeks after operation,Masson staining was used to observe the histopathology of myocardial infarction region,and the protein expressions of Gal-3,collagen Ⅰ and Ⅲ were detected by Western blot.Results After 4 weeks of administration,the heart function of the MCP group was significantly improved compared with the cardiac insufficiency group (P＜0.01).The serum Gal-3 in cardiac insufficiency group was higher than that in sham operation group (P＜0.05),and serum Gal-3 in MCP group was significantly lower than that in cardiac insufficiency group (P＜0.05).Correlation analysis showed that left ventricular ejection fraction was negatively correlated with serum Gal-3 (r=-0.841,P=0.009),and left ventricular end-diastolic diameter was positively correlated with serum Gal-3 (r=0.905,P=0.002),in cardiac insufficiency group.Compared with cardiac insufficiency group,the mRNA expression of Gal-3,collagen Ⅰ and Ⅲ in MCP group was significandy decreased (P＜0.05).Under optical microscope,cardiac insufficiency group showed myocardial cell disorder,part of myocardial cell necrosis,edema,acollagen deposition and inflammatory cell infiltration;MCP group showed myocardial fiber thickening,arranging in a slightly tidy order.Protein expressions of Gal-3,collagen Ⅰ and Ⅲ in the infarcted myocardium of cardiac insufficiency group were significantly higher than those in MCP group and sham operation group.Correlation analysis showed that Gal-3 in infarction region was positively correlated with collagen Ⅲ in cardiac insufficiency group (r=0.793,P=0.019).Conclusion Gal-3 plays a key role in the progressions of ventricular remodeling and myocardial fibrosis in heart failure.关键词
半乳糖凝聚素3/改良柑橘果胶/心肌纤维化/心室重构Key words
Galectin-3/Modified citrus pectin/Myocardial fibrosis/Ventricular remodeling分类
医药卫生
