中国动脉硬化杂志2017，Vol.25Issue(10)：1021-1024,4.
宁夏回汉族As患者外周血中ICAM-1基因DNA甲基化与血脂水平的关系
The relationship between ICAM-1 DNA methylation and serum lipid level in Hui and Han race patients with atherosclerosis of Ningxia
摘要
Abstract
Aim To study the relationship between DNA methylation level of intercellular adhesion molecule 1 (ICAM-1) and atherosclerosis (As) occurrence,development and changes of blood lipids.Methods The atherosclerosis patients (100 patients from Hui and Han ethnic with As) were confirmed by carotid artery Doppler ultrasound and the serum lipids were measured by ADVIA 2400 automatic biochemistry analyzer,ICAM-1 mRNA expression was determined by real-time reverse transcription-polymerase chain reaction (qRT-PCR).The methylation status of ICAM-1 gene promoter region was examined using nest touch down methylation specificpolymerase chain reaction (nt-MSP).Results Compared with As patients from Han ethnic,ICAM-1 mRNA expression levels were promoted in Hui ethnic (P＜0.001),and the levels of ICAM-1 promoter methylation was decreased in Hui ethnic (P＜0.001).Compared with As patients from Hui ethnic,triglycerides (TG) concentrations was promoted in As patients from Han ethnic(P ＜0.001),and was negatively correlated with ICAM-1 promoter methylation (r=-0.5783,P＜0.001).Conclusion ICAM-1 DNA hypomethylation is related to the occurrence and development of As and the changes of serum lipids in peripheral blood mononuclear cells of Hui patients.关键词
细胞间黏附分子1/DNA甲基化/动脉粥样硬化/血脂Key words
Intercellular adhesion molecule 1/DNA methylation/Atherosclerosis/Blood lipids分类
医药卫生引用本文复制引用
吴凯,马胜超,徐灵博,张辉,王艳华,杨晓玲,姜怡邓,沈江涌..宁夏回汉族As患者外周血中ICAM-1基因DNA甲基化与血脂水平的关系[J].中国动脉硬化杂志,2017,25(10):1021-1024,4.基金项目
宁夏高等学校科学研究项目(NGY2016086) （NGY2016086）
