利用M基因鉴别新城疫病毒中强毒株与弱毒株RT-nPCR方法的建立OA北大核心CSTPCD
ESTABLISHMENT OF RT-NPCR METHOD FOR IDENTIFICATION OF VELOGENIC AND LENTOGENIC NEWCASTLE DISEASE VIRUS STRAINS BY USING M GENE
根据GenBank上公开的新城疫病毒(Newcastle disease virus,NDV)基因序列,针对M基因差异位点设计了3对引物,通过优化反应体系与条件,建立了能够区分NDV中强毒株与弱毒株的RT-nPCR检测方法.特异性试验显示,NDV中强毒株扩增出543、375 bp两个条带,弱毒株仅出现375 bp一个条带,其他常见禽病毒为阴性.灵敏度试验结果表明,该方法检测cDNA含量极限为6.15×10-4 ng/μL.临床样品检测发现鸡群ND…查看全部>>
According to the Newcastle disease virus(NDV) gene sequence published in GenBank, three pairs of primers were designed for M gene difference sites of NDV, we changed reaction system and conditions until PCR reaction was optimal. The results of sensitivity test showed that the limit of cDNA content was 6.15×10-4 ng/μL. Specificity tests showed that 543 bp and 375 bp bands were found in the velogenic NDV strains, and only 375 bp bands were found in the l…查看全部>>
朱小甫;吴旭锦
咸阳职业技术学院畜牧兽医研究所动物疫病分子生物学诊断实验室,咸阳 712000咸阳职业技术学院畜牧兽医研究所动物疫病分子生物学诊断实验室,咸阳 712000
农业科技
新城疫病毒中强毒株弱毒株M基因鉴别诊断
Newcastle disease virusvelogenic strainslentogenic strainsM genedifferential diagnosis
《中国动物传染病学报》 2017 (5)
21-25,5
咸阳职业技术学院重大科研项目(2016KYA01)
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