中国动物传染病学报2017，Vol.25Issue(5)：64-67,4.
基于12S rRNA基因序列的犬弓首蛔虫种系发育关系分析
PHYLOGENETIC ANALYSIS OF MITOCHONDRIAL 12S rRNA GENE OF TOXOCARA CANLSIN HENAN PROVINCE
摘要
Abstract
The aims of the present study were to analyze sequence variations in the mitochondrial 12S rRNA gene among Toxocara canls isolates from Henan province. The 12S rRNA was amplified from each of Toxocara canls isolates by PCR and analyzed using the ClustalX 2.0. The results showed that the lengths of all the 12S rRNA sequences were 497 - 499 bp. The 12S rRNA sequences of the Henan isolates and reference Toxocara canls available in GenBank were aligned for phylogenetic analysis and found that they all clustered in the same clade. Therefore, 12S rRNA sequence could be used as a genetic marker for population genetic studies of ascarid. The results of the present study provided foundation for further studies of diagnosis and molecular epidemiology of Toxocara canls.关键词
犬弓首蛔虫/线粒体DNA/12SrRNA基因/种系发育关系Key words
Toxocara canls/mitochondrial DNA/12S rRNA gene/phylogenetic relationship分类
农业科技引用本文复制引用
项黎丽,苏玉贤..基于12S rRNA基因序列的犬弓首蛔虫种系发育关系分析[J].中国动物传染病学报,2017,25(5):64-67,4.基金项目
平顶山市科技发展计划项目(20160315-N02) （20160315-N02）
