中国动物传染病学报2017，Vol.25Issue(5)：68-72,5.
弓形虫胶体金免疫层析试纸条的研制
DEVELOPMENT OF COLLOIDAL GOLD IMMUNOCHROMATOGRAPHIC STRIP ASSAY FOR TOXOPLASMOSIS DIAGNOSIS
摘要
Abstract
In order to develop a colloidal gold immunochromatographic strip assay for toxoplasmosis diagnosis, the surface antigen genes of SAG1 and SAG2 of Toxoplasma gondiiwere synthesized and subcloned into plasmid pET-28a(+) to be expressed in Escherichia coliBL21(DE3). To obtain high concentrations of recombinant SAGs, the target proteins were purified using His-fusion protein purification kit and analyzed in Western blotting. Recombinant SAG1 and SAG2 were blotted on the nitrocellulose membrane and murine T. gondiipositive and negative serum samples were then added as primary antibodies to test the sensitivity of colloidal gold immune chromatography strip. Development of the method laid a foundation for rapid diagnosis of toxoplasmosis at the grass-roots levels.关键词
胶体金免疫层析试纸条/重组蛋白/弓形虫Key words
Colloidal gold immunochromatographic strip/recombinant protein/Toxoplasma分类
农业科技引用本文复制引用
王钊哲,江嘉欣,李嘉静,朱传刚,许瑞,洪炀,林矫矫,陆珂,李浩,陈兆国,唐亚兰,吴思敏..弓形虫胶体金免疫层析试纸条的研制[J].中国动物传染病学报,2017,25(5):68-72,5.基金项目
国家重点研发计划(2017YFD0501306) （2017YFD0501306）
中央级公益性科研院所基本科研业务费(2017JB20) （2017JB20）
