中国兽医科学2017，Vol.47Issue(10)：1214-1220,7.DOI:10.16656/j.issn.1673-4696.2017.10.002
非洲猪瘟病毒抗体量子点检测试纸条的研制
Establishment of a quantum dots-based immunochromatographic strip for detection of the antibodies against African swine fever virus
摘要
Abstract
According to the amino acid sequence of the African swine fever virus(ASFV) VP73 protein,the antigen epitope dominant region was predicted and compounded,and then conjugated with bovine serum albumin(BSA).The polypeptide was immobilized on the detection zone of the immunochromatographic strip,and the rabbit anti-protein A was immobilized on the control zone.The water-soluble quantum dots(QDs) were used as the signal output and conjugated to streptococcal protein A (SPA),which was capable of binding to immunoglobulin G(IgG) from many species through an amide bond to capture the target anti-ASFV IgG.The results showed that the immunochromatographic strip was specific for ASFV-positive sera.No cross-reactions were observed for the positive sera against other porcine viruses.The coincidence rate between the strip and a commercial ELISA kit was 100％.The strip is specific,sensitive,stable,and easy to operate.It can be used for rapid detection of anti-ASFV antibodies and epidemiological investigation.关键词
非洲猪瘟病毒/VP73基因/量子点/免疫层析试纸条Key words
African swine fever virus/VP73 gene/quantum dots/immunochromatographic strip分类
农业科技引用本文复制引用
林彦星,吕建强,花群义,曹琛福,张彩虹,孙洁,杨俊兴,廖立珊,黄超华,刘建利,阮周曦..非洲猪瘟病毒抗体量子点检测试纸条的研制[J].中国兽医科学,2017,47(10):1214-1220,7.基金项目
“十三五”国家重点研发计划课题项目(2016YFD0500708) （2016YFD0500708）
国家质检总局科技项目(2016IK233) （2016IK233）
深圳检验检疫局科技项目(SZ2015217) （SZ2015217）
