中国兽医科学2017，Vol.47Issue(10)：1227-1233,7.DOI:10.16656/j.issn.1673-4696.2017.10.004
田鼠巴贝斯虫醛酮还原酶BmAKR2的基因克隆和鉴定
Gene cloning and characterization of aldo-keto reductase BmAKR2 from Babesia microti
摘要
Abstract
In order to understand antioxidant ability of Babesia microti in oxidative stress environment,a novel aldo-keto reductase(AKR) gene was cloned from B.microti and identified.The full-length BmAKR2 cDNA contained an open reading frame of 2 490 bp in length,which encoded an 829 amino acid polypep tide.A classic AKRs conserved domainwas found in the 172nd to 542nd amino acid residue by BLAST analysis.The conserved domain was subcloned into the pGEX-4T-1 expression vector,and the protein was expressed as a glutathione S-transferase(GST)-fusion protein in Escherichia coli BL21 (DE3) cells.Recombinant BmAKR2-HX protein was injected into mice to produce antibodies.The result of Western-blot showed a native BmAKR weight was approximately 96 ku that was similar to the predicted value.The result of indirect immunofluorescence assay showed that BmAKR2 was located in the nuclei of B.microti merozoites.The results of real time RT-PCR showed BmAKR2 mRNA was transcribed on 2-14 days post infection(DPI),but was highest on 5 DPI.关键词
田鼠巴贝斯虫/醛酮还原酶/克隆/鉴定Key words
Babesia microti/aldo-keto reductase/cloning/characterization分类
农业科技引用本文复制引用
黄强,曹杰,周勇志,龚海燕,张厚双,周金林..田鼠巴贝斯虫醛酮还原酶BmAKR2的基因克隆和鉴定[J].中国兽医科学,2017,47(10):1227-1233,7.基金项目
国家重点基础研究发展计划(973)项目(2015CB150300) （973）
