中国兽医科学2017，Vol.47Issue(10)：1234-1239,6.DOI:10.16656/j.issn.1673-4696.2017.10.005
猪源奇异变形杆菌的分离鉴定及其毒力的测定
Isolation, identification and toxicity test of Proteus mirabilis from swine
摘要
Abstract
To investigate the biological characteristics,drug resistance and virulence of the pathogen which caused the death of weaned pigs,an isolate was identified through bacteria morphology,physiological and biochemical assays,the sequence analysis of 16S rDNA,drug susceptibility test and median lethal dose(LD50) test.The results showed that the isolate was Proteus mirabilis.The analysis of phylogenetic tree showed that the isolate was highly similar to the P.mirabilis CIFRI P-TSB-13 (JF784025) strain which was separated from Indian seawater.Drug susceptibility test indicated the isolate was highly sensitive to cefoxitin,ceftazidime,spectinomycin and aztreonam,moderately sensitive to 10 kinds of antibiotic including minocycline,amikacin and nitrofurantoin,etc.,and fully resistant to 21 kinds of antibiotic including gentamycin,ofloxacin and levofloxacin,etc.,indicating that the isolate had multi-drug resistance.When zebrafish were cloacal injected and mice were intraperitoneally injected with the strain,it had high virulence as measured by the LD50 of 2.12 × 105 CFU/mL and 1.46 × 107 CFU/mL.This research confirmed that the pathogen of the diseased pigs was P.mirabilis,and suggested to use the highly sensitive antibiotic drugs on clinical treatment.关键词
猪/奇异变形杆菌/16S rDNA/耐药性/半数致死量Key words
pig/Proteus mirabilis/16S rDNA/drug resistance/median lethal dose分类
农业科技引用本文复制引用
马婷婷,罗廷荣,韦显凯,闭璟珊,易驰喆,苏姣秀,巫介棚,梁晟,张步娴,郑敏..猪源奇异变形杆菌的分离鉴定及其毒力的测定[J].中国兽医科学,2017,47(10):1234-1239,6.基金项目
广西水产畜牧兽医科技推广应用项目(桂渔牧科201633001) （桂渔牧科201633001）
公益性行业(农业)科研专项(201303041) （农业）
