中国兽医科学2017，Vol.47Issue(10)：1240-1244,5.DOI:10.16656/j.issn.1673-4696.2017.10.006
壳聚糖季铵盐凝乳胶微球对O型口蹄疫VLPs免疫豚鼠的免疫增强作用
Immunological enhancement of the quaternized chitosan-gel microspheres on immunization of virus-like particles of foot-and-mouth disease virus serotype O in guinea pigs
摘要
Abstract
To study the immune effects of the quaternized chitosan-gel microspheres on the FMDV virus-like particles(VLPs),we used the VLPs of serotype O FMDV as antigens emulsified by the chitosan microspheres(Chitosan A) or white oil chitosan microspheres(Chitosan B) to immunize guinea pigs.Tlymphocyte proliferation,FMDV specific antibodies and the protection rate of virus challenge were detected and evaluated.In result,the quaternized chitosan-gel microspheres promoted high levels of specific FMDV antibodies and T lymphocyte proliferation in guinea pigs,which was higher than that of ISA201.In addition,the result showed that Chitosan B adjuvant was better than Chitosan A adjuvant.In conclusion,the quaternized chitosan-gel microspheres can enhance immune effects of FMDV VLPs and induce both humoral and cellular immune responses.The study confirmed the quaternized chitosan-gel microspheres could be a potential vaccine adjuvant and become a new candidate of FMDV vaccine adjuvant.关键词
壳聚糖季铵盐凝乳胶微球/口蹄疫病毒/病毒样颗粒/佐剂Key words
ouaternized Chitosan-gel microspheres/foot-and-mouth disease virus (FMDV)/virus-like particles (VLPs)/adjuvant分类
农业科技引用本文复制引用
朱向涛,孙世琪,吴颉,郭慧琛,马军武..壳聚糖季铵盐凝乳胶微球对O型口蹄疫VLPs免疫豚鼠的免疫增强作用[J].中国兽医科学,2017,47(10):1240-1244,5.基金项目
国家自然科学基金项目(31672592) （31672592）
中央级科研院所基本科研业务费专项(1610312016002,1610312017010) （1610312016002,1610312017010）
中国农业科学院青年英才计划项目 （）
